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anti-Human TEK antibody for Intracellular Staining

Recommended TEK Antibody (supplied by: Log in to see )

Antigen
TEK Tyrosine Kinase, Endothelial (TEK) Antibodies
  • AA517024
  • CD202B
  • Cd202b
  • DKFZp469E064
  • Hyk
  • TEK
  • Tie-2
  • TIE-2
  • tie-2
  • TIE2
  • tie2
  • Tie2
  • VMCM
  • VMCM1
Epitope
pTyr992
29
25
23
22
21
19
17
14
14
14
13
10
7
6
6
5
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
336
151
74
6
6
2
1
Host
Mouse
196
145
34
3
2
2
Clonality
Monoclonal
Conjugate
This TEK antibody is conjugated to Alexa Fluor 647
25
19
16
13
12
11
9
8
6
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
2
1
Application
Intracellular Staining (ICS)
240
185
135
71
49
48
48
39
20
11
9
7
4
3
3
2
1
Supplier
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Catalog No. ABIN1177217
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000870507 ABIN1177218 ICS PE Mouse IgG2a, kappa pTyr1102 Log in to see K93-754 6
0.000870507 ABIN1177219 ICS Alexa Fluor 647 Mouse IgG2a, kappa pTyr1102 Log in to see K93-754 6
0.000870507 ABIN1177220 ICS Alexa Fluor 488 Mouse IgG2a, kappa pTyr1102 Log in to see K93-754 6

Top referenced anti-TEK antibody for Intracellular Staining

Similar anti-TEK Antibodies

Application / Reactivity Human Mouse (Murine)
Simple Western (SimWes) 2 Antibodies 2 Antibodies
Western Blotting (WB) 214 Antibodies 73 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies
Intracellular Staining (ICS) 4 Antibodies 4 Antibodies
Immunoprecipitation (IP) 6 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies 36 Antibodies
Immunohistochemistry (IHC) 56 Antibodies 33 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 49 Antibodies 49 Antibodies
Immunofluorescence (IF) 21 Antibodies 29 Antibodies

General

Antigen TEK Tyrosine Kinase, Endothelial (TEK) Antibodies
Epitope pTyr992
(29), (25), (23), (22), (21), (19), (17), (14), (14), (14), (13), (10), (7), (6), (6), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(336), (151), (74), (6), (6), (2), (1)
Host Mouse
(196), (145), (34), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TEK antibody is conjugated to Alexa Fluor 647
(25), (19), (16), (13), (12), (11), (9), (8), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (1)
Application Intracellular Staining (ICS)
(240), (185), (135), (71), (49), (48), (48), (39), (20), (11), (9), (7), (4), (3), (3), (2), (1)
Pubmed 6 references available
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Product Details anti-TEK Antibody

Target Details TEK Application Details Handling References for anti-TEK Antibody (ABIN1177217) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human TIE2 Peptide
Clone K91-860
Isotype IgG1, kappa

Target Details TEK

Product Details anti-TEK Antibody Application Details Handling References for anti-TEK Antibody (ABIN1177217) Images back to top
Antigen
Alternative Name TIE2 (TEK Antibody Abstract)
Background TIE2 (Tyrosine kinase with Immunoglobulin-like and EGF-like domains 2), also known as TEK (Tunical Endothelial Kinase), is an endothelial and hematopoietic cell-specific receptor tyrosine kinase (RTK) that is critical to the development and maintenance of the vasculature and highly conserved among vertebrate species. The angiopoietins are ligands of TIE2, and the abnormal vascular growth that often occurs in solid tumors is a result of disruptions in the coordinated actions of the angiopoietins, TIE2, and the closely related TIE1 RTK. Upon activation by angiopoietins, TIE2 autophosphorylates at least 2 tyrosines in its protein kinase domain and at least 3 tyrosines in its C-terminal tail. The K91-860 monoclonal antibody recognizes the phosphorylated tyrosine 992 (Y992) in the protein kinase domain of activated TIE2. The orthologous phosphorylation site in mouse TIE2 is Y990.
Synonyms: TEK, VMCM, CD202b
Research Area Tyrosine Kinases
Pathways RTK Signaling

Application Details

Product Details anti-TEK Antibody Target Details TEK Handling References for anti-TEK Antibody (ABIN1177217) Images back to top
Application Notes Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation.
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-TEK Antibody Target Details TEK Application Details References for anti-TEK Antibody (ABIN1177217) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-TEK Antibody (ABIN1177217)

Product Details anti-TEK Antibody Target Details TEK Application Details Handling Images back to top
Product cited in:

Audero, Cascone, Maniero, Napione, Arese, Lanfrancone, Bussolino: "Adaptor ShcA protein binds tyrosine kinase Tie2 receptor and regulates migration and sprouting but not survival of endothelial cells." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 13224-33, 2004

Peters, Kontos, Lin, Wong, Rao, Huang, Dewhirst, Sankar: "Functional significance of Tie2 signaling in the adult vasculature." in: Recent progress in hormone research, Vol. 59, pp. 51-71, 2004

DeBusk, Hallahan, Lin: "Akt is a major angiogenic mediator downstream of the Ang1/Tie2 signaling pathway." in: Experimental cell research, Vol. 298, Issue 1, pp. 167-77, 2004

Jones, Iljin, Dumont, Alitalo: "Tie receptors: new modulators of angiogenic and lymphangiogenic responses." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 4, pp. 257-67, 2001

Murray, Padrique, Pinko, McTigue: "Mechanistic effects of autophosphorylation on receptor tyrosine kinase catalysis: enzymatic characterization of Tie2 and phospho-Tie2." in: Biochemistry, Vol. 40, Issue 34, pp. 10243-53, 2001

Edgell, McDonald, Graham: "Permanent cell line expressing human factor VIII-related antigen established by hybridization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 12, pp. 3734-7, 1983