anti-Zebrafish TEK antibody for Western Blotting

Recommended TEK Antibody (supplied by: Log in to see )

Antigen
TEK Tyrosine Kinase, Endothelial (TEK) Antibodies
  • TEK
  • DKFZp469E064
  • AA517024
  • Cd202b
  • Hyk
  • Tie2
  • tie-2
  • CD202B
  • TIE-2
  • TIE2
  • VMCM
  • VMCM1
  • Tie-2
  • tie2
  • TEK tyrosine kinase, endothelial
  • endothelial-specific receptor tyrosine kinase
  • TEK
  • LOC100356675
  • tek
  • Tek
Reactivity
Zebrafish
367
179
85
7
6
2
1
Host
Goat
204
169
48
3
3
2
Clonality
Polyclonal
Conjugate
This TEK antibody is un-conjugated
29
21
19
17
14
13
9
9
8
7
7
6
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
4
2
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
266
194
140
135
64
53
43
33
22
13
8
7
5
5
4
4
4
2
1
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Catalog No. ABIN956714
$ 793.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2629013 WB Rabbit pTyr1108 Log in to see Polyclonal

General

Antigen TEK Tyrosine Kinase, Endothelial (TEK) Antibodies
Reactivity Zebrafish
(367), (179), (85), (7), (6), (2), (1)
Host Goat
(204), (169), (48), (3), (3), (2)
Clonality
Polyclonal
Conjugate This TEK antibody is un-conjugated
(29), (21), (19), (17), (14), (13), (9), (9), (8), (7), (7), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (2)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(266), (194), (140), (135), (64), (53), (43), (33), (22), (13), (8), (7), (5), (5), (4), (4), (4), (2), (1)
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Product Details anti-TEK Antibody

Target Details TEK Application Details Handling Images
Specificity Zebrafish Tie-2.
Purification Immunoaffinity purified
Sterility 0.2 μm filtered
Immunogen NS0-derived rzfTie-2 extracellular domain.

Type of Immunogen: Recombinant protein
Isotype IgG

Target Details TEK

Product Details anti-TEK Antibody Application Details Handling Images back to top
Antigen
Alternative Name TEK / TIE2 (TEK Antibody Abstract)
Background Name/Gene ID: TEK
Subfamily: Tie/Tek
Family: Protein Kinase

Synonyms: TEK, Angiopoietin-1 receptor, CD202b antigen, CD202B, Endothelial tyrosine kinase, HTIE2, Hyk, Soluble TIE2 variant 1, Soluble TIE2 variant 2, TIE-2, TIE2, VMCM, Tek tyrosine kinase, VMCM1, p140 TEK
Gene ID 7010
UniProt Q02763
Pathways RTK Signaling, Growth Factor Binding

Application Details

Product Details anti-TEK Antibody Target Details TEK Handling Images back to top
Application Notes Approved: ELISA (50 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Blocking of receptor-ligand interaction-Approximately 4 to 10 μg/mL of this antibody will block 50 % of the binding of 25 ng/mL of biotinylated rhAng-2 to immobilized rzfTie-2/Fc chimera (100 μL of a 4 μg/mL solution was coated in each well) in a functional ELISA. Approximately 50 μg/mL of this antibody will block 95 % of the binding. Western blot-This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect zebrafish Tie-2. The detection limit for rzfTie-2 is approximately 2 ng/lane under non-reducing and reducing conditions. Immunohistochemistry-This antibody has been used at a concentration of 10 μg/mL to detect Tie2 in 24hpf zebrafish embryos. Dechorionated zebrafish embryos were fixed overnight in 4 % paraformaldehyde at 4° C and then dehydrated and rehydrated through a series of methanol washes. Embryos were blocked in PBS containing 0.1 % Tween 20, 2 % normal donkey serum, and 1 % BSA for 2 hours at room temperature. After blocking, embryos were incubated in diluted primary antibody overnight at 4 °C followed by. donkey anti-goat IgG at room temperature for 2 hours. Between each step, embryos were washed in PBS containing 0.1 % Tween 20. Direct ELISA-This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect zebrafish Tie-2. The detection limit for rzfTie-2 is approximately. 0.1 ng/well.
Restrictions For Research Use only

Handling

Product Details anti-TEK Antibody Target Details TEK Application Details Images back to top
Format Lyophilized
Concentration Lot specific
Buffer Lyophilized from 0.2 μm filtered 100 mM NaHCO3, pH 8.3, 500 mM NaCl, 1 % sucrose.
Storage -20 °C
Storage Comment Store at -20°C.

Images

Product Details anti-TEK Antibody Target Details TEK Application Details Handling back to top
Supplier Images
 image for anti-TEK Tyrosine Kinase, Endothelial (TEK) antibody (ABIN956714) anti-TEK Tyrosine Kinase, Endothelial (TEK) antibody