|Antigen||Vimentin (VIM) Antibodies|
|Reactivity||All Species Alternatives|
|Conjugate||This Vimentin antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-Vimentin AntibodyTarget Details Vimentin Application Details Handling ProductDetails: References for anti-Vimentin antibody (ABIN120733) Images
|Specificity||The antibody reacts with Vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types in all species (recognized epitope conserved within all species). Cross-reactivity was found with smooth muscle Desmin.|
|Immunogen||Pellet of pig brain cold stable proteins after depolymerization of microtubules|
Target Details VimentinProduct Details anti-Vimentin Antibody Application Details Handling ProductDetails: References for anti-Vimentin antibody (ABIN120733) Images back to top
|Alternative Name||Vimentin (VIM Antibody Abstract)|
|Background||Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin. Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.Synonyms: VIM|
|Research Area||Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers|
|Pathways||Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Vimentin Antibody Target Details Vimentin Handling ProductDetails: References for anti-Vimentin antibody (ABIN120733) Images back to top
Western Blotting. Immunocytochemistry: Staining technique: RBL rat basophilic leukemia cell line:(a) Fix cells for 10 min in methanol at - 20 °C and for 6 min in acetone at -20 °C, (b) Fix cells directly in methanol for 10 min at -20 °C or in acetone for 10 min at -20 °C. Incubation: 45 min RT, Positive control: 3T3 mouse Swiss albino fibroblast cell line RBL rat basophilic leukemia cell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details ProductDetails: References for anti-Vimentin antibody (ABIN120733) Images back to top
|Buffer||PBS , pH ~7.4, 15 mM Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody at 2 - 8 °C. Do not freeze!
Shelf Life: one year from despatch.
|Expiry Date||12 months|
ProductDetails: References for anti-Vimentin antibody (ABIN120733)Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
|Product cited in:||
Bacáková, Mares, Bottone, Pellicciari, Lisá, Svorcík: "Fluorine ion-implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture." in: Journal of biomedical materials research, Vol. 49, Issue 3, pp. 369-79, 2000
Bacáková, Mares, Lisá, Svorcík: "Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions." in: Biomaterials, Vol. 21, Issue 11, pp. 1173-9, 2000
Lukás, Dráber, Bucek, Dráberová, Viklický, Dolezel: "Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord." in: Histochemistry, Vol. 100, Issue 6, pp. 495-502, 1994