You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Cow (Bovine) Vimentin antibody for Immunofluorescence

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Reactivity
Human, Cow (Bovine), Pig (Porcine), Mouse (Murine), Rat (Rattus), Mammalian
576
165
147
72
52
42
26
25
15
10
9
9
8
6
5
4
4
3
2
2
1
1
1
1
1
1
1
Host
Mouse
310
261
30
12
11
6
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
23
21
13
10
9
5
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
477
237
185
142
122
85
84
52
47
23
15
11
8
8
3
3
3
3
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1580467
$ 220.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.31611317 ABIN1580468 ICC IF IHC WB Mouse IgG2a Log in to see 2D1 2
0.31611317 ABIN2705329 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.31611317 ABIN1741989 IF WB Mouse Log in to see 2D1
0.31611317 ABIN2493867 IF WB Mouse Log in to see
0.31611317 ABIN1741988 IF WB Chicken Log in to see Polyclonal
0.0010850475 ABIN112306 EIA IHC (fro) IF IHC (p) WB Mouse IgG2a Log in to see VIM 3B4 10
0.0010850475 ABIN112308 EIA FACS IHC (fro) IF IHC (p) WB FITC Mouse IgG2a Log in to see VIM 3B4 11
0.0010850475 ABIN967290 IF IHC WB Mouse IgG1 Log in to see RV202 4
0.0010850475 ABIN284838 IHC (fro) IF IHC (p) ELISA WB Mouse IgG2a Log in to see VIM 3B4
0.0010850475 ABIN289202 IHC (fro) IF IHC (p) ELISA WB FITC Mouse IgG2a Log in to see VIM 3B4
0.0010850475 ABIN345779 IF ELISA Mouse IgG2a Log in to see VIM 3B4
0.0010850475 ABIN488097 IF WB Chicken Ig Log in to see Polyclonal
0.0010850475 ABIN222825 IHC (fro) IF IHC (p) ELISA WB FITC Mouse IgG2a Log in to see
0.0010850475 ABIN225111 IHC (fro) IF IHC (p) ELISA WB Mouse IgG2a pSer72 Log in to see
0.0010850475 ABIN2385500 IF IHC ELISA WB Mouse IgG2a Log in to see 5F310
0.0010850475 ABIN2385499 IF IHC ELISA WB Mouse IgG2a Log in to see 2Q1035
0.0010850475 ABIN1582223 IF ELISA WB Chicken Log in to see Polyclonal
0.0010850475 ABIN3026782 FACS IF IHC WB Mouse IgG1 Log in to see VM452
0.0010850475 ABIN933237 IF IHC ELISA WB Mouse IgG2a Log in to see VIM 3B4
0.0010850475 ABIN2385502 IF IHC ELISA WB FITC Mouse IgG2a Log in to see 5F310

Top referenced anti-Vimentin antibody for Immunofluorescence

Similar anti-Vimentin Antibodies

Application / Reactivity Mammalian Human Pig (Porcine) Mouse (Murine) Rat (Rattus) Cow (Bovine)
Western Blotting (WB) 8 Antibodies 449 Antibodies 47 Antibodies 125 Antibodies 134 Antibodies 42 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Luminex Assay (LMNX) 8 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Immunoprecipitation (IP) 42 Antibodies 12 Antibodies 8 Antibodies 16 Antibodies 1 Antibodies 1 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human, Cow (Bovine), Pig (Porcine), Mouse (Murine), Rat (Rattus), Mammalian
(576), (165), (147), (72), (52), (42), (26), (25), (15), (10), (9), (9), (8), (6), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(310), (261), (30), (12), (11), (6), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(23), (21), (13), (10), (9), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(477), (237), (185), (142), (122), (85), (84), (52), (47), (23), (15), (11), (8), (8), (3), (3), (3), (3), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN1580467) Images
Purification affinity purified antibody
Clone 2A52
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN1580467) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin is a protein subunit of 10 nm or intermediate filaments, which are major components of the cytoskeleton in most cell types. Vimentin is the major intemediate filament subunit found in mesenchymal cells, and was first named and characterized in a collaborative study from the labs of German scientists Werner Franke and Klaus Weber. The name derives from the Latin Vimentum, meaning arrays of flexible rods such as in lattices, filigrees and wicker-work, which describes the intermediate filament network quite well. Vimentin is also found in many cell types in tissue culture, most notably fibroblasts, and in developing neuronal and astrocytic precursor cells in the central nervous system. Many cell lines such as Hek293, HeLa, 3T3 and Cos cells contain prominent vimentin networks. Vimentin frequently forms copolymers with other intermediate filament proteins, such as GFAP (in astrocytes, ependymal cells and neural stem cells), with desmin (in muscle and endothelial cells) and neurofilament proteins (in developing neurons). A E151K point mutation in the vimentin gene was shown to be causative of an autosomal dominant pulverulent cataract disease, but so far only in a single patient. Vimentin is a major protein of eye lens and cornea, and this mutation renders the molecule unable assemble into normal 10 nm filaments. Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. The immunogen used to generate our antibody was recombinant human vimentin expressed in and purified from E. coli. The same immunogen was used to produce our other monoclonal antibody to vimentin MCA-2D1. We also market a very popular chicken polyclonal antibody to vimentin, CPCA-Vim. The HGNC name for this protein is VIM.
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN1580467) Images back to top
Application Notes The antibody solution can be used at dilutions of at least 1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10,000 or lower.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN1580467) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer The antibody solution is affinity purified from tissue culture supernatant and is at concentration of 1mg/ml in phosphate buffered saline preparation containing 10 mM sodium azide preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 4°C short term or -20°C long term.

References for anti-Vimentin Antibody (ABIN1580467)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Müller, Bhattacharya, Moore, Prescott, Wedig, Herrmann, Magin: "Dominant cataract formation in association with a vimentin assembly disrupting mutation." in: Human molecular genetics, Vol. 18, Issue 6, pp. 1052-7, 2009

Franke, Schmid, Osborn, Weber: "Different intermediate-sized filaments distinguished by immunofluorescence microscopy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 75, Issue 10, pp. 5034-8, 1979

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN1580467) back to top
Supplier Images
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (ABIN1580467) Western blot of crude extract of the human carcinoma HeLa cell line. Lane 18 was prob...
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (ABIN1580467) View of mixed neuron/glial cultures stained with CPCA-Vim (green) and ’s rabbit antib...