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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Chicken||Cow (Bovine)|
|Immunofluorescence (fixed cells) (IF/ICC)||13 Antibodies||8 Antibodies||8 Antibodies||2 Antibodies||1 Antibodies|
|Cytometry by Time of Flight (CyTOF)||10 Antibodies||4 Antibodies||5 Antibodies||3 Antibodies||2 Antibodies|
|Simple Western (SimWes)||5 Antibodies||5 Antibodies||4 Antibodies||2 Antibodies|
|Western Blotting (WB)||666 Antibodies||235 Antibodies||274 Antibodies||97 Antibodies||103 Antibodies|
|Transfected Cell Culture (TCC)||1 Antibodies|
|Antigen||Vimentin (VIM) Antibodies|
|Reactivity||Chicken, Cow (Bovine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Vimentin antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-Vimentin AntibodyTarget Details Vimentin Application Details Handling Images
|Specificity||Recognizes Vimentin (50-55 kDa).|
|Immunogen||Synthetic peptide surrounding amino acid 448 of Human Vimentin.|
Target Details VimentinProduct Details anti-Vimentin Antibody Application Details Handling Images back to top
|Alternative Name||Vimentin (VIM Antibody Abstract)|
|Background||Vimentin, a intermediate filament protein, is a general marker of cells originating in the mesenchyme. Vimentin is frequently co-expressed with other members of the intermediate filament family such as the cytokeratins, in neoplasm.Synonyms: VIM|
|Research Area||Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers|
|Pathways||Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Vimentin Antibody Target Details Vimentin Handling Images back to top
Western Blot: 0.5-4 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunohistochemistry: 10-20 μg/mL. Positive Control: Mouse small intestine tissue lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details Images back to top
|Buffer||Phosphate-buffered saline (PBS) containing 30 % Glycerol, 0.5 % BSA as stabilizer and 0.01 % Thimerosal as preservative|
|Precaution of Use||This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C/-80 °C|
|Storage Comment||Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C to -70 °C.|
ImagesProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling back to top