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anti-Hamster Vimentin antibody for Western Blotting

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Goat, Hamster, Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus)
576
165
147
73
52
41
25
24
14
11
9
8
7
6
5
4
4
3
2
2
1
1
1
1
1
1
1
Host
Mouse
310
261
30
12
11
6
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
23
21
13
10
9
5
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
477
237
185
142
122
85
85
52
47
23
15
11
8
8
3
3
3
3
1
1
1
Supplier
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Catalog No. ABIN967290
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011806369 ABIN112354 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see RV202 5
0.0011806369 ABIN126094 IHC (fro) IHC (p) WB Guinea Pig Log in to see Polyclonal 2
0.0011806369 ABIN335272 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV203 1
0.0011806369 ABIN335271 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
0.0011806369 ABIN335380 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
0.0011806369 ABIN335381 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV203 1
0.0011806369 ABIN1042494 ICC IHC (fro) FACS IHC (p) WB HRP Mouse IgG1 Log in to see RV202 5
0.0011806369 ABIN1805484 ICC IHC (fro) WB HRP Mouse IgG1 Log in to see RV202

Top referenced anti-Vimentin antibody for Western Blotting

Similar anti-Vimentin Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Cow (Bovine) Monkey Dog (Canine) Goat Hamster Rabbit
Western Blotting (WB) 449 Antibodies 125 Antibodies 134 Antibodies 33 Antibodies 42 Antibodies 21 Antibodies 20 Antibodies 13 Antibodies 9 Antibodies 4 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Luminex Assay (LMNX) 8 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Immunoprecipitation (IP) 42 Antibodies 8 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Chicken, Cow (Bovine), Dog (Canine), Goat, Hamster, Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus)
(576), (165), (147), (73), (52), (41), (25), (24), (14), (11), (9), (8), (7), (6), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(310), (261), (30), (12), (11), (6), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(23), (21), (13), (10), (9), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(477), (237), (185), (142), (122), (85), (85), (52), (47), (23), (15), (11), (8), (8), (3), (3), (3), (3), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN967290) Images
Brand BD Pharmingen™
Cross-Reactivity Rat (Rattus), Mouse (Murine), Hamster, Chicken, Rabbit, Goat, Dog (Canine), Monkey, Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Purified Cow Lens Vimentin
Clone RV202
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN967290) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Intermediate filaments (IF) are a subset of cytoskeletal proteins which function to give overall structural integrity to the plasma membrane as well to organize cells into specific tissues. IF proteins can be divided into six major types based upon the similarity in sequence. Vimentin belongs to the type III category of IF proteins which are expressed in leukocytes, blood vessel endothelial cells, some epithelial cells, and mesenchymal cells. Vimentin is also expressed together with several other IF proteins during the early stages of development. Vimentin is exchanged for the tissue-specific intermediate filament type as differentiation proceeds. Vimentin migrates in SDS/PAGE as a ~57 kDa protein.
Molecular Weight 57 kDa
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN967290) Images back to top
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN967290) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-Vimentin Antibody (ABIN967290)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990

Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, 1989

Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, 1988

Background publications

Broers, Carney, Klein Rot, Schaart, Lane, Vooijs, Ramaekers: "Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies." in: Journal of cell science, Vol. 83, Issue 12, pp. 37-60, 1987

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN967290) back to top
Supplier Images
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (ABIN967290) Western blot analysis of vimentin. HeLa cell lysate was probed with anti-vimentin (cl...