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anti-Human Vimentin antibody for Flow Cytometry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Reactivity
Human
797
291
244
207
121
118
103
79
78
43
40
32
31
27
26
7
5
4
3
2
2
1
1
1
1
1
1
Host
Rabbit
523
267
30
12
12
7
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
28
26
21
18
13
13
11
11
11
11
11
11
11
11
11
11
9
5
5
5
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
666
294
289
288
240
227
218
131
104
23
15
11
8
8
5
3
3
3
3
1
1
1
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Catalog No. ABIN1686955
$ 464.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30379152 ABIN94494 ICC FACS ELISA WB Mouse IgG1 Log in to see VI-RE-1 1
0.30379152 ABIN192298 FACS PE Mouse IgG1 Log in to see VI-RE-1 1
0.30379152 ABIN1558082 ICC FACS ELISA WB APC Mouse IgG1 Log in to see VI-RE-1 1
0.30379152 ABIN1881993 FACS IF IHC WB Rabbit Log in to see Polyclonal 3
0.30379152 ABIN269144 ICC FACS IF ELISA WB Mouse IgG1 Log in to see VI-RE-1
0.30379152 ABIN1574034 FACS IHC ELISA WB Rabbit IgG AA 400-500 Log in to see Polyclonal
0.0010485103 ABIN112354 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see RV202 5
0.0010485103 ABIN389213 FACS IHC (p) WB Rabbit Ig AA 152-181, Center Log in to see Polyclonal 3
0.0010485103 ABIN389212 FACS IF IHC (p) WB Rabbit Ig AA 430-457, C-Term Log in to see Polyclonal 4
0.0010485103 ABIN112184 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see V9 5
0.0010485103 ABIN112307 EIA FACS IHC (fro) IHC (p) Biotin Mouse IgG2a Log in to see VIM 3B4 12
0.0010485103 ABIN112308 EIA FACS IHC (fro) IF IHC (p) WB FITC Mouse IgG2a Log in to see VIM 3B4 11
0.0010485103 ABIN1981416 FACS IF IHC WB Rabbit IgG AA 430-457, C-Term Log in to see Polyclonal
0.0010485103 ABIN959503 ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see RV202
0.0010485103 ABIN959504 ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see RV203
0.0010485103 ABIN959513 ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see V9
0.0010485103 ABIN335272 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV203 1
0.0010485103 ABIN335273 ICC FACS IHC (fro) IHC (p) IHC WB Mouse IgG1 Log in to see V9 7
0.0010485103 ABIN335271 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
0.0010485103 ABIN204914 ICC FACS IHC (fro) IHC (p) WB Mouse IgG1 pSer72 Log in to see

Top referenced anti-Vimentin antibody for Flow Cytometry

Similar anti-Vimentin Antibodies

Application / Reactivity Human
ELISA 211 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 217 Antibodies
Immunochromatography (IC) 12 Antibodies
Immunocytochemistry (ICC) 240 Antibodies
Immunodiffusion (ID) 3 Antibodies
Immunoelectrophoresis (IEP) 3 Antibodies
Immunofluorescence (IF) 283 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 280 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 119 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 281 Antibodies
Immunoprecipitation (IP) 99 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Luminex Assay (LMNX) 8 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human
(797), (291), (244), (207), (121), (118), (103), (79), (78), (43), (40), (32), (31), (27), (26), (7), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(523), (267), (30), (12), (12), (7), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(28), (26), (21), (18), (13), (13), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (9), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(666), (294), (289), (288), (240), (227), (218), (131), (104), (23), (15), (11), (8), (8), (5), (3), (3), (3), (3), (1), (1), (1)
Pubmed 20 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN1686955) Images
Predicted Reactivity Cow (Bovine),Chicken,Frog,Human,Mouse (Murine),Rat (Rattus)
Characteristics Cellular Localization: Cytoplasm
Immunogen Recombinant human vimentin protein.
Clone SP20
Isotype IgG

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN1686955) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin is the main intermediate filament protein in mesenchymal cells. This protein is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin is expressed in sarcomas of neural, muscle and fibroblast origin, but carcinomas are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin.
Molecular Weight 53 kDa
Gene ID 7431
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN1686955) Images back to top
Application Notes IHC Dilution: 1:200
Comment

Positive Control:
IHC (p): Melanomas, sarcomas
WB: HeLa Cell Lysate

Protocol IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: None
Primary Antibody Incubation: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN1686955) Images back to top
Format Liquid
Buffer rabbit monoclonal antibody supplied as tissue culture supernatant in TBS/1 % BSA buffer pH 7.5 with less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Storage 4 °C

References for anti-Vimentin Antibody (ABIN1686955)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Li, Zhang, Han, Ouyang: "Plexiform myxoid gastrointestinal stromal tumor: a potential diagnostic pitfall in pathological findings." in: International journal of clinical and experimental pathology, Vol. 8, Issue 10, pp. 13613-8, 2016

Harkness, Zaher, Ditzel, Isa, Kassem: "CD146/MCAM defines functionality of human bone marrow stromal stem cell populations." in: Stem cell research & therapy, Vol. 7, pp. 4, 2016

Speranza, Nowicki, Behera, Cho, Chiocca, Lawler: "BKM-120 (Buparlisib): A Phosphatidyl-Inositol-3 Kinase Inhibitor with Anti-Invasive Properties in Glioblastoma." in: Scientific reports, Vol. 6, pp. 20189, 2016

Caceres, Peña, de Andres, Illera, Lopez, Woodward, Reuben, Illera: "Establishment and characterization of a new cell line of canine inflammatory mammary cancer: IPC-366." in: PLoS ONE, Vol. 10, Issue 3, pp. e0122277, 2015

Wu, Li, Liu: "Epithelioid angiosarcoma: a clinicopathological study of 16 Chinese cases." in: International journal of clinical and experimental pathology, Vol. 8, Issue 4, pp. 3901-9, 2015

Mineshige, Sugahara, Ohmuro, Kamiie, Shirota: "Lymphangiosarcoma with bone formation of the auricle in a dog." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 77, Issue 6, pp. 739-42, 2015

Bhagirath, Zhao, West, Qiu, Band, Band: "Cell type of origin as well as genetic alterations contribute to breast cancer phenotypes." in: Oncotarget, Vol. 6, Issue 11, pp. 9018-30, 2015

Åkerfelt, Bayramoglu, Robinson, Toriseva, Schukov, Härmä, Virtanen, Sormunen, Kaakinen, Kannala, Eklund, Heikkilä, Nees: "Automated tracking of tumor-stroma morphology in microtissues identifies functional targets within the tumor microenvironment for therapeutic intervention." in: Oncotarget, Vol. 6, Issue 30, pp. 30035-56, 2015

Jennings, Carroll: "Oligodendrocyte Lineage Cells in Chronic Demyelination of Multiple Sclerosis Optic Nerve." in: Brain pathology (Zurich, Switzerland), Vol. 25, Issue 5, pp. 517-30, 2015

Moser, Megerle, Bekos, Janik, Szerafin, Birner, Schiefer, Mildner, Lang, Skoro-Sajer, Sadushi-Kolici, Taghavi, Klepetko, Ankersmit: "Local and systemic RAGE axis changes in pulmonary hypertension: CTEPH and iPAH." in: PLoS ONE, Vol. 9, Issue 9, pp. e106440, 2014

Huang, Zhang, Cai, Shi, Ke, Dong, Zhang, Fan, Peng, Zhou: "Comprehensive multiple molecular profile of epithelial mesenchymal transition in intrahepatic cholangiocarcinoma patients." in: PLoS ONE, Vol. 9, Issue 5, pp. e96860, 2014

Joannes, Grelet, Duca, Gilles, Kileztky, Dalstein, Birembaut, Polette, Nawrocki-Raby: "Fhit regulates EMT targets through an EGFR/Src/ERK/Slug signaling axis in human bronchial cells." in: Molecular cancer research : MCR, Vol. 12, Issue 5, pp. 775-83, 2014

Wang, Bajikar, Jamal, Atkins, Janes: "A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies." in: Nature cell biology, Vol. 16, Issue 4, pp. 345-56, 2014

Walker, Gopalakrishnan, Bogyo, Basilion: "Microscopic detection of quenched activity-based optical imaging probes using an antibody detection system: localizing protease activity." in: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, Vol. 16, Issue 5, pp. 608-18, 2014

Treloar, Simpson, Haridas, Manton, Leavesley, McElwain, Baker: "Multiple types of data are required to identify the mechanisms influencing the spatial expansion of melanoma cell colonies." in: BMC systems biology, Vol. 7, pp. 137, 2013

Haug, Azqueta, Johnsen-Soriano, Shahdadfar, Drolsum, Moe, Røger, Romero, Collins, Nicolaissen: "Donor cornea transfer from Optisol GS to organ culture storage: a two-step procedure to increase donor tissue lifespan." in: Acta ophthalmologica, Vol. 91, Issue 3, pp. 219-25, 2013

Jambal, Badtke, Harrell, Borges, Post, Sollender, Spillman, Horwitz, Jacobsen: "Estrogen switches pure mucinous breast cancer to invasive lobular carcinoma with mucinous features." in: Breast cancer research and treatment, Vol. 137, Issue 2, pp. 431-48, 2013

Mace, Ameen, Collins, Wojcik, Mair, Young, Fuchs, Eubank, Frankel, Bekaii-Saab, Bloomston, Lesinski: "Pancreatic cancer-associated stellate cells promote differentiation of myeloid-derived suppressor cells in a STAT3-dependent manner." in: Cancer research, Vol. 73, Issue 10, pp. 3007-18, 2013

Burden-Gulley, Zhou, Craig, Lu, Brady-Kalnay: "Molecular Magnetic Resonance Imaging of Tumors with a PTPµ Targeted Contrast Agent." in: Translational oncology, Vol. 6, Issue 3, pp. 329-37, 2013

Gaiffe, Prétet, Launay, Jacquin, Saunier, Hetzel, Oudet, Mougin: "Apoptotic HPV positive cancer cells exhibit transforming properties." in: PLoS ONE, Vol. 7, Issue 5, pp. e36766, 2012

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN1686955) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Vimentin antibody (VIM) (ABIN1686955) Flow cytometric analysis of rabbit anti-Vimentin (SP20) antibody in HeLa (green) comp...
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (ABIN1686955) anti-Vimentin (VIM) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (ABIN1686955) anti-Vimentin (VIM) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (ABIN1686955) anti-Vimentin (VIM) antibody (Image 4)
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (ABIN1686955) anti-Vimentin (VIM) antibody (Image 5)
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (ABIN1686955) anti-Vimentin (VIM) antibody (Image 6)
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (ABIN1686955) anti-Vimentin (VIM) antibody (Image 7)
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (ABIN1686955) anti-Vimentin (VIM) antibody (Image 8)
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (ABIN1686955) anti-Vimentin (VIM) antibody (Image 9)
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (ABIN1686955) anti-Vimentin (VIM) antibody (Image 10)
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (ABIN1686955) anti-Vimentin (VIM) antibody (Image 11)
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (ABIN1686955) anti-Vimentin (VIM) antibody (Image 12)
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (ABIN1686955) anti-Vimentin (VIM) antibody (Image 13)