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|Application / Reactivity||Human|
|Blocking Antibody (Inhibition)||1 Antibodies|
|Cytometry by Time of Flight (CyTOF)||10 Antibodies|
|Enzyme Immunoassay (EIA)||8 Antibodies|
|Flow Cytometry (FACS)||223 Antibodies|
|Immunochromatography (IC)||12 Antibodies|
|Immunocytochemistry (ICC)||211 Antibodies|
|Immunodiffusion (ID)||3 Antibodies|
|Immunoelectrophoresis (IEP)||3 Antibodies|
|Immunofluorescence (IF)||260 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||13 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||13 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||40 Antibodies|
|Immunohistochemistry (IHC)||263 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||104 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||280 Antibodies|
|Antigen||Vimentin (VIM) Antibodies|
|Conjugate||This Vimentin antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|20 references available|
|Supplier||Log in to see|
Product Details anti-Vimentin AntibodyTarget Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN1686955) Images
|Predicted Reactivity||Cow (Bovine),Chicken,Frog,Human,Mouse (Murine),Rat (Rattus)|
|Characteristics||Cellular Localization: Cytoplasm|
|Immunogen||Recombinant human vimentin protein.|
Target Details VimentinProduct Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN1686955) Images back to top
|Alternative Name||Vimentin (VIM Antibody Abstract)|
|Background||Vimentin is the main intermediate filament protein in mesenchymal cells. This protein is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin is expressed in sarcomas of neural, muscle and fibroblast origin, but carcinomas are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin.|
|Molecular Weight||53 kDa|
|Research Area||Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers|
|Pathways||TCR Signaling, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN1686955) Images back to top
|Application Notes||IHC Dilution: 1:200|
IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: None
Primary Antibody Incubation: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
|Restrictions||For Research Use only|
HandlingProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN1686955) Images back to top
|Buffer||rabbit monoclonal antibody supplied as tissue culture supernatant in TBS/1 % BSA buffer pH 7.5 with less than 0.1 % sodium azide.|
|Precaution of Use||
1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
|Handling Advice||Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.|
References for anti-Vimentin Antibody (ABIN1686955)Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
|Product cited in:||
Li, Zhang, Han, Ouyang: "Plexiform myxoid gastrointestinal stromal tumor: a potential diagnostic pitfall in pathological findings." in: International journal of clinical and experimental pathology, Vol. 8, Issue 10, pp. 13613-8, 2016
Harkness, Zaher, Ditzel, Isa, Kassem: "CD146/MCAM defines functionality of human bone marrow stromal stem cell populations." in: Stem cell research & therapy, Vol. 7, pp. 4, 2016
Speranza, Nowicki, Behera, Cho, Chiocca, Lawler: "BKM-120 (Buparlisib): A Phosphatidyl-Inositol-3 Kinase Inhibitor with Anti-Invasive Properties in Glioblastoma." in: Scientific reports, Vol. 6, pp. 20189, 2016
Caceres, Peña, de Andres, Illera, Lopez, Woodward, Reuben, Illera: "Establishment and characterization of a new cell line of canine inflammatory mammary cancer: IPC-366." in: PLoS ONE, Vol. 10, Issue 3, pp. e0122277, 2015
Wu, Li, Liu: "Epithelioid angiosarcoma: a clinicopathological study of 16 Chinese cases." in: International journal of clinical and experimental pathology, Vol. 8, Issue 4, pp. 3901-9, 2015
Mineshige, Sugahara, Ohmuro, Kamiie, Shirota: "Lymphangiosarcoma with bone formation of the auricle in a dog." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 77, Issue 6, pp. 739-42, 2015
Bhagirath, Zhao, West, Qiu, Band, Band: "Cell type of origin as well as genetic alterations contribute to breast cancer phenotypes." in: Oncotarget, Vol. 6, Issue 11, pp. 9018-30, 2015
Åkerfelt, Bayramoglu, Robinson, Toriseva, Schukov, Härmä, Virtanen, Sormunen, Kaakinen, Kannala, Eklund, Heikkilä, Nees: "Automated tracking of tumor-stroma morphology in microtissues identifies functional targets within the tumor microenvironment for therapeutic intervention." in: Oncotarget, Vol. 6, Issue 30, pp. 30035-56, 2015
Jennings, Carroll: "Oligodendrocyte Lineage Cells in Chronic Demyelination of Multiple Sclerosis Optic Nerve." in: Brain pathology (Zurich, Switzerland), Vol. 25, Issue 5, pp. 517-30, 2015
Moser, Megerle, Bekos, Janik, Szerafin, Birner, Schiefer, Mildner, Lang, Skoro-Sajer, Sadushi-Kolici, Taghavi, Klepetko, Ankersmit: "Local and systemic RAGE axis changes in pulmonary hypertension: CTEPH and iPAH." in: PLoS ONE, Vol. 9, Issue 9, pp. e106440, 2014
Huang, Zhang, Cai, Shi, Ke, Dong, Zhang, Fan, Peng, Zhou: "Comprehensive multiple molecular profile of epithelial mesenchymal transition in intrahepatic cholangiocarcinoma patients." in: PLoS ONE, Vol. 9, Issue 5, pp. e96860, 2014
Joannes, Grelet, Duca, Gilles, Kileztky, Dalstein, Birembaut, Polette, Nawrocki-Raby: "Fhit regulates EMT targets through an EGFR/Src/ERK/Slug signaling axis in human bronchial cells." in: Molecular cancer research : MCR, Vol. 12, Issue 5, pp. 775-83, 2014
Wang, Bajikar, Jamal, Atkins, Janes: "A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies." in: Nature cell biology, Vol. 16, Issue 4, pp. 345-56, 2014
Walker, Gopalakrishnan, Bogyo, Basilion: "Microscopic detection of quenched activity-based optical imaging probes using an antibody detection system: localizing protease activity." in: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, Vol. 16, Issue 5, pp. 608-18, 2014
Treloar, Simpson, Haridas, Manton, Leavesley, McElwain, Baker: "Multiple types of data are required to identify the mechanisms influencing the spatial expansion of melanoma cell colonies." in: BMC systems biology, Vol. 7, pp. 137, 2013
Haug, Azqueta, Johnsen-Soriano, Shahdadfar, Drolsum, Moe, Røger, Romero, Collins, Nicolaissen: "Donor cornea transfer from Optisol GS to organ culture storage: a two-step procedure to increase donor tissue lifespan." in: Acta ophthalmologica, Vol. 91, Issue 3, pp. 219-25, 2013
Jambal, Badtke, Harrell, Borges, Post, Sollender, Spillman, Horwitz, Jacobsen: "Estrogen switches pure mucinous breast cancer to invasive lobular carcinoma with mucinous features." in: Breast cancer research and treatment, Vol. 137, Issue 2, pp. 431-48, 2013
Mace, Ameen, Collins, Wojcik, Mair, Young, Fuchs, Eubank, Frankel, Bekaii-Saab, Bloomston, Lesinski: "Pancreatic cancer-associated stellate cells promote differentiation of myeloid-derived suppressor cells in a STAT3-dependent manner." in: Cancer research, Vol. 73, Issue 10, pp. 3007-18, 2013
Burden-Gulley, Zhou, Craig, Lu, Brady-Kalnay: "Molecular Magnetic Resonance Imaging of Tumors with a PTPµ Targeted Contrast Agent." in: Translational oncology, Vol. 6, Issue 3, pp. 329-37, 2013
Gaiffe, Prétet, Launay, Jacquin, Saunier, Hetzel, Oudet, Mougin: "Apoptotic HPV positive cancer cells exhibit transforming properties." in: PLoS ONE, Vol. 7, Issue 5, pp. e36766, 2012
ImagesProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN1686955) back to top