anti-Monkey Vimentin antibody for ELISA

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Epitope
AA 2-466
40
25
24
22
20
18
15
15
14
8
7
7
6
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey
928
406
356
221
138
132
121
83
81
52
50
43
41
37
30
12
8
7
5
5
4
3
3
2
2
1
1
1
1
1
Host
Mouse
569
321
30
17
12
9
9
6
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
29
27
26
23
18
16
13
13
13
13
13
13
13
13
12
11
7
7
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
739
345
327
305
254
252
250
144
87
40
15
14
13
10
8
7
5
3
3
3
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN969454
$ 442.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2869381 ELISA IHC WB Mouse IgG1 AA 2-466 Log in to see 4F2E9 2
1 ABIN1501715 ELISA IHC (p) WB Mouse IgG1 Log in to see 9E7E7-
1 ABIN4365304 ELISA IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 4F2E9 1
1 ABIN1824101 ICC IHC ELISA WB Mouse IgG2a Log in to see VIM 3B4
1 ABIN222825 IHC (fro) IF IHC (p) ELISA WB FITC Mouse IgG2a Log in to see
1 ABIN225111 IHC (fro) IF IHC (p) ELISA WB Mouse IgG2a Log in to see
1 ABIN5621544 ELISA IHC WB Mouse IgG1 AA 2-466 Log in to see 4F2E9
1 ABIN1846715 IHC ELISA WB Mouse IgG1 AA 2-466 Log in to see
1 ABIN1582223 IF ELISA WB Chicken Log in to see Polyclonal
1 ABIN4365303 ELISA IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 4F2E9
1 ABIN4902452 ELISA IHC WB Mouse IgG1 Log in to see 4F2E9
1 ABIN1580212 IHC ELISA WB Mouse Log in to see

General

Antigen Vimentin (VIM) Antibodies
Epitope AA 2-466
(40), (25), (24), (22), (20), (18), (15), (15), (14), (8), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey
(928), (406), (356), (221), (138), (132), (121), (83), (81), (52), (50), (43), (41), (37), (30), (12), (8), (7), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(569), (321), (30), (17), (12), (9), (9), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(29), (27), (26), (23), (18), (16), (13), (13), (13), (13), (13), (13), (13), (13), (12), (11), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(739), (345), (327), (305), (254), (252), (250), (144), (87), (40), (15), (14), (13), (10), (8), (7), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN969454) Images
Purification Ascites
Immunogen Purified recombinant fragment of Vimentin (aa2-466) expressed in E. Coli.
Clone 4F2E9
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN969454) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin, also know as VIM. It is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Molecular Weight 54 kDa
Gene ID 7431
HGNC 7431
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN969454) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN969454) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-Vimentin antibody (ABIN969454)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Yates, Zetterberg, Rajeev, Reiss, Rittling: "Promoter-independent regulation of vimentin expression in mammary epithelial cells by val(12)ras and TGFbeta." in: Experimental cell research, Vol. 313, Issue 17, pp. 3718-28, 2007

Gilles, Polette, Mestdagt, Nawrocki-Raby, Ruggeri, Birembaut, Foidart: "Transactivation of vimentin by beta-catenin in human breast cancer cells." in: Cancer research, Vol. 63, Issue 10, pp. 2658-64, 2003

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN969454) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (AA 2-466) antibody (ABIN969454) Figure 1: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tiss...
Western Blotting (WB) image for anti-Vimentin (VIM) (AA 2-466) antibody (ABIN969454) Figure 2: Western blot analysis using Vimentin mouse mAb against Hela (1), COS (2), H...