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anti-Monkey Vimentin antibody for ELISA

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Epitope
AA 2-466
24
18
17
16
16
15
15
14
13
12
8
8
7
5
4
4
4
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey
840
314
273
206
121
111
95
77
75
42
39
32
30
28
25
6
6
4
3
3
2
1
1
1
1
1
1
Host
Mouse
527
303
30
15
12
8
7
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
28
28
23
20
15
14
12
11
11
11
11
11
11
11
11
9
5
5
5
3
3
3
3
3
3
3
3
3
3
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
660
296
270
269
243
223
218
108
82
40
15
13
10
8
8
5
3
3
3
3
1
1
1
1
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Catalog No. ABIN969454
$ 442.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2869381 ELISA IHC WB Mouse IgG1 AA 2-466 Log in to see 4F2E9 2
1 ABIN4365304 ELISA IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 4F2E9 1
1 ABIN2501134 IHC ELISA WB Mouse IgG1 Log in to see 9E7E7-5G3F10-5G3F10E5
1 ABIN1846715 IHC ELISA WB Mouse IgG1 AA 2-466 Log in to see
1 ABIN1582223 IF ELISA WB Chicken Log in to see Polyclonal
1 ABIN4365303 ELISA IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 4F2E9
1 ABIN4902452 ELISA IHC WB Mouse IgG1 Log in to see 4F2E9
1 ABIN1580212 IHC ELISA WB Mouse Log in to see

Top referenced anti-Vimentin antibody for ELISA

Similar anti-Vimentin Antibodies

Application / Reactivity Monkey Human
ELISA 9 Antibodies 226 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 8 Antibodies
Flow Cytometry (FACS) 8 Antibodies 214 Antibodies
Immunochromatography (IC) 2 Antibodies 12 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 212 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunohistochemistry (IHC) 14 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 12 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies
Western Blotting (WB) 25 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies
Immunodiffusion (ID) 3 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Epitope AA 2-466
(24), (18), (17), (16), (16), (15), (15), (14), (13), (12), (8), (8), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey
(840), (314), (273), (206), (121), (111), (95), (77), (75), (42), (39), (32), (30), (28), (25), (6), (6), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(527), (303), (30), (15), (12), (8), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(28), (28), (23), (20), (15), (14), (12), (11), (11), (11), (11), (11), (11), (11), (11), (9), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(660), (296), (270), (269), (243), (223), (218), (108), (82), (40), (15), (13), (10), (8), (8), (5), (3), (3), (3), (3), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN969454) Images
Purification Ascites
Immunogen Purified recombinant fragment of Vimentin (AA 2-466) expressed in E. coli.
Clone 4F2E9
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN969454) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin, also know as VIM. It is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Molecular Weight 54 kDa
Gene ID 7431
HGNC 7431
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN969454) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN969454) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-Vimentin Antibody (ABIN969454)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Yates, Zetterberg, Rajeev, Reiss, Rittling: "Promoter-independent regulation of vimentin expression in mammary epithelial cells by val(12)ras and TGFbeta." in: Experimental cell research, Vol. 313, Issue 17, pp. 3718-28, 2007

Gilles, Polette, Mestdagt, Nawrocki-Raby, Ruggeri, Birembaut, Foidart: "Transactivation of vimentin by beta-catenin in human breast cancer cells." in: Cancer research, Vol. 63, Issue 10, pp. 2658-64, 2003

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN969454) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (AA 2-466) (ABIN969454) Figure 1: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tiss...
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (AA 2-466) (ABIN969454) Figure 2: Western blot analysis using Vimentin mouse mAb against Hela (1), COS (2), H...