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anti-Monkey Vimentin antibody for ELISA

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Epitope
AA 2-466
21
16
16
15
14
14
13
12
12
8
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey
578
168
149
74
54
42
26
24
15
11
9
9
8
6
5
4
4
3
2
2
1
1
1
1
1
1
1
Host
Mouse
312
261
30
12
11
6
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
23
21
14
10
9
5
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
479
237
184
142
125
87
85
52
47
23
15
11
8
8
3
3
3
3
1
1
1
Supplier
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Catalog No. ABIN969454
$ 442.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012127591 ABIN2501134 IHC ELISA WB Mouse IgG1 Log in to see 9E7E7-5G3F10-5G3F10E5
0.0012127591 ABIN1846715 IHC ELISA WB Mouse IgG1 AA 2-466 Log in to see
0.0012127591 ABIN1582223 IF ELISA WB Chicken Log in to see Polyclonal
0.0012127591 ABIN1580212 IHC ELISA WB Mouse Log in to see

Top referenced anti-Vimentin antibody for ELISA

Similar anti-Vimentin Antibodies

Application / Reactivity Monkey Human
ELISA 5 Antibodies 169 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 8 Antibodies
Flow Cytometry (FACS) 8 Antibodies 83 Antibodies
Immunochromatography (IC) 2 Antibodies 12 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 87 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunohistochemistry (IHC) 10 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Western Blotting (WB) 21 Antibodies
Immunodiffusion (ID) 3 Antibodies
Immunoelectrophoresis (IEP) 3 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Epitope AA 2-466
(21), (16), (16), (15), (14), (14), (13), (12), (12), (8), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey
(578), (168), (149), (74), (54), (42), (26), (24), (15), (11), (9), (9), (8), (6), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(312), (261), (30), (12), (11), (6), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(23), (21), (14), (10), (9), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(479), (237), (184), (142), (125), (87), (85), (52), (47), (23), (15), (11), (8), (8), (3), (3), (3), (3), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN969454) Images
Purification Ascites
Immunogen Purified recombinant fragment of Vimentin (AA 2-466) expressed in E. coli.
Clone 4F2E9
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN969454) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin, also know as VIM. It is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Molecular Weight 54 kDa
Gene ID 7431
HGNC 7431
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN969454) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN969454) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-Vimentin Antibody (ABIN969454)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Yates, Zetterberg, Rajeev, Reiss, Rittling: "Promoter-independent regulation of vimentin expression in mammary epithelial cells by val(12)ras and TGFbeta." in: Experimental cell research, Vol. 313, Issue 17, pp. 3718-28, 2007

Gilles, Polette, Mestdagt, Nawrocki-Raby, Ruggeri, Birembaut, Foidart: "Transactivation of vimentin by beta-catenin in human breast cancer cells." in: Cancer research, Vol. 63, Issue 10, pp. 2658-64, 2003

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN969454) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (AA 2-466) (ABIN969454) Figure 1: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tiss...
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (AA 2-466) (ABIN969454) Figure 2: Western blot analysis using Vimentin mouse mAb against Hela (1), COS (2), H...