You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Monkey Vimentin antibody for Western Blotting

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Epitope
AA 2-466
21
16
16
15
14
14
13
12
12
8
5
4
4
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey
576
166
148
73
53
42
26
24
15
11
9
9
8
6
5
4
4
3
2
2
1
1
1
1
1
1
1
Host
Mouse
310
261
30
12
11
6
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
23
21
13
10
9
5
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
477
237
184
142
123
85
85
52
47
23
15
11
8
8
3
3
3
3
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN969454
$ 442.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12581924 ABIN967290 IF IHC WB Mouse IgG1 Log in to see RV202 4
0.12581924 ABIN2705329 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.12581924 ABIN2707781 WB Rabbit N-Term, pSer56 Log in to see Polyclonal
0.12581924 ABIN2707782 IC IF WB Rabbit N-Term Log in to see Polyclonal
0.12581924 ABIN1109483 IHC (p) WB Mouse IgG1 Log in to see 4F2E9 1
0.12581924 ABIN2501134 IHC ELISA WB Mouse IgG1 Log in to see 9E7E7-5G3F10-5G3F10E5
0.12581924 ABIN1846715 IHC ELISA WB Mouse IgG1 AA 2-466 Log in to see
0.0011810986 ABIN112354 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see RV202 5
0.0011810986 ABIN112306 EIA IHC (fro) IF IHC (p) WB Mouse IgG2a Log in to see VIM 3B4 10
0.0011810986 ABIN112308 EIA FACS IHC (fro) IF IHC (p) WB FITC Mouse IgG2a Log in to see VIM 3B4 11
0.0011810986 ABIN335271 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
0.0011810986 ABIN335380 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
0.0011810986 ABIN1042494 ICC IHC (fro) FACS IHC (p) WB HRP Mouse IgG1 Log in to see RV202 5
0.0011810986 ABIN1042493 ICC IHC (fro) FACS IHC (p) WB FITC Mouse IgG1 Log in to see RV202 5
0.0011810986 ABIN1805484 ICC IHC (fro) WB HRP Mouse IgG1 Log in to see RV202
0.0011810986 ABIN3223017 IHC WB Rabbit C-Term Log in to see Polyclonal
0.0011810986 ABIN1583532 IHC WB Mouse IgG1 AA 2-466 Log in to see 4F2E9
0.0011810986 ABIN1582223 IF ELISA WB Chicken Log in to see Polyclonal
0.0011810986 ABIN2972291 ICC IF WB Rabbit N-Term Log in to see Polyclonal
0.0011810986 ABIN1580212 IHC ELISA WB Mouse Log in to see

Top referenced anti-Vimentin antibody for Western Blotting

Similar anti-Vimentin Antibodies

Application / Reactivity Monkey Human
ELISA 5 Antibodies 169 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 8 Antibodies
Flow Cytometry (FACS) 8 Antibodies 83 Antibodies
Immunochromatography (IC) 2 Antibodies 12 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 85 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunohistochemistry (IHC) 10 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Western Blotting (WB) 21 Antibodies
Immunodiffusion (ID) 3 Antibodies
Immunoelectrophoresis (IEP) 3 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Epitope AA 2-466
(21), (16), (16), (15), (14), (14), (13), (12), (12), (8), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey
(576), (166), (148), (73), (53), (42), (26), (24), (15), (11), (9), (9), (8), (6), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(310), (261), (30), (12), (11), (6), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(23), (21), (13), (10), (9), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(477), (237), (184), (142), (123), (85), (85), (52), (47), (23), (15), (11), (8), (8), (3), (3), (3), (3), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN969454) Images
Purification Ascites
Immunogen Purified recombinant fragment of Vimentin (AA 2-466) expressed in E. coli.
Clone 4F2E9
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN969454) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin, also know as VIM. It is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Molecular Weight 54 kDa
Gene ID 7431
HGNC 7431
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN969454) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN969454) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-Vimentin Antibody (ABIN969454)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Yates, Zetterberg, Rajeev, Reiss, Rittling: "Promoter-independent regulation of vimentin expression in mammary epithelial cells by val(12)ras and TGFbeta." in: Experimental cell research, Vol. 313, Issue 17, pp. 3718-28, 2007

Gilles, Polette, Mestdagt, Nawrocki-Raby, Ruggeri, Birembaut, Foidart: "Transactivation of vimentin by beta-catenin in human breast cancer cells." in: Cancer research, Vol. 63, Issue 10, pp. 2658-64, 2003

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN969454) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (AA 2-466) (ABIN969454) Figure 1: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tiss...
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (AA 2-466) (ABIN969454) Figure 2: Western blot analysis using Vimentin mouse mAb against Hela (1), COS (2), H...