You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Monkey Vimentin antibody for Western Blotting

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Epitope
AA 2-466
22
21
18
16
16
16
16
15
12
9
7
6
6
6
5
4
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey
838
364
311
219
137
132
114
83
79
50
47
41
41
36
30
10
7
6
5
5
4
3
3
2
2
1
1
1
1
1
Host
Mouse
518
295
29
14
12
8
7
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
27
27
23
20
15
14
12
11
11
11
11
11
11
11
11
9
5
5
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
665
288
284
265
237
221
216
107
82
40
15
14
13
10
8
8
5
3
3
3
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN969454
$ 442.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0726016 ABIN967290 IF IHC WB Mouse IgG1 Log in to see RV202 4
3.0726016 ABIN2707782 IC IF WB Rabbit N-Term Log in to see Polyclonal
3.0726016 ABIN2707781 WB Rabbit N-Term, pSer56 Log in to see Polyclonal
3.0726016 ABIN2705329 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
3.0726016 ABIN1109483 IHC (p) WB Mouse IgG1 Log in to see 4F2E9 1
3.0726016 ABIN3223017 IHC WB Rabbit C-Term Log in to see Polyclonal
3.0726016 ABIN1846715 IHC ELISA WB Mouse IgG1 AA 2-466 Log in to see
1 ABIN112306 EIA IHC (fro) IF IHC (p) WB Mouse IgG2a Log in to see VIM 3B4 10
1 ABIN112354 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see RV202 5
1 ABIN112308 EIA FACS IHC (fro) IF IHC (p) WB FITC Mouse IgG2a Log in to see VIM 3B4 11
1 ABIN2869381 ELISA IHC WB Mouse IgG1 AA 2-466 Log in to see 4F2E9 2
1 ABIN321407 WB Rabbit IgG AA 110-159 Log in to see Polyclonal
1 ABIN604756 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN959503 ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see RV202
1 ABIN335271 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
1 ABIN1501715 ELISA IHC (p) WB Mouse IgG1 Log in to see 9E7E7-
1 ABIN4365304 ELISA IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 4F2E9 1
1 ABIN335380 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
1 ABIN1042493 ICC IHC (fro) FACS IHC (p) WB FITC Mouse IgG1 Log in to see RV202 5
1 ABIN1042494 ICC IHC (fro) FACS IHC (p) WB HRP Mouse IgG1 Log in to see RV202 5

Top referenced anti-Vimentin antibody for Western Blotting

Similar anti-Vimentin Antibodies

Application / Reactivity Monkey Human
ELISA 12 Antibodies 224 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 8 Antibodies
Flow Cytometry (FACS) 9 Antibodies 217 Antibodies
Immunochromatography (IC) 2 Antibodies 12 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunohistochemistry (IHC) 16 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Western Blotting (WB) 41 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Epitope AA 2-466
(22), (21), (18), (16), (16), (16), (16), (15), (12), (9), (7), (6), (6), (6), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey
(838), (364), (311), (219), (137), (132), (114), (83), (79), (50), (47), (41), (41), (36), (30), (10), (7), (6), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(518), (295), (29), (14), (12), (8), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(27), (27), (23), (20), (15), (14), (12), (11), (11), (11), (11), (11), (11), (11), (11), (9), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(665), (288), (284), (265), (237), (221), (216), (107), (82), (40), (15), (14), (13), (10), (8), (8), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN969454) Images
Purification Ascites
Immunogen Purified recombinant fragment of Vimentin (AA 2-466) expressed in E. coli.
Clone 4F2E9
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN969454) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin, also know as VIM. It is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Molecular Weight 54 kDa
Gene ID 7431
HGNC 7431
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN969454) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN969454) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-Vimentin Antibody (ABIN969454)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Yates, Zetterberg, Rajeev, Reiss, Rittling: "Promoter-independent regulation of vimentin expression in mammary epithelial cells by val(12)ras and TGFbeta." in: Experimental cell research, Vol. 313, Issue 17, pp. 3718-28, 2007

Gilles, Polette, Mestdagt, Nawrocki-Raby, Ruggeri, Birembaut, Foidart: "Transactivation of vimentin by beta-catenin in human breast cancer cells." in: Cancer research, Vol. 63, Issue 10, pp. 2658-64, 2003

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN969454) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (AA 2-466) (ABIN969454) Figure 1: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tiss...
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (AA 2-466) (ABIN969454) Figure 2: Western blot analysis using Vimentin mouse mAb against Hela (1), COS (2), H...