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anti-Mouse (Murine) Vimentin antibody for Flow Cytometry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Epitope
AA 400-500
23
17
16
16
16
15
14
13
12
8
7
7
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus), Mouse (Murine), Human
806
293
253
205
118
110
95
77
75
42
39
32
30
29
25
7
5
4
3
2
2
1
1
1
1
1
1
Host
Rabbit
519
277
30
15
12
7
5
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
27
26
23
18
14
13
12
11
11
11
11
11
11
11
11
9
5
5
5
4
3
2
2
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
671
299
290
290
239
233
231
128
104
25
15
12
8
8
5
3
3
3
3
1
1
1
Supplier
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Catalog No. ABIN1574034
$ 147.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.28290245 ABIN4896119 FACS Allophycocyanin Rat IgG2a AA 2-466 Log in to see 280618
0.28290245 ABIN4896120 FACS Alexa Fluor 488 Rat IgG2a AA 2-466 Log in to see 280618
0.28290245 ABIN4896121 FACS PE Rat IgG2a AA 2-466 Log in to see 280618
0.0010564557 ABIN112354 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see RV202 5
0.0010564557 ABIN672786 FACS IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 416-466 Log in to see Polyclonal 23
0.0010564557 ABIN335271 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
0.0010564557 ABIN335380 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
0.0010564557 ABIN1042493 ICC IHC (fro) FACS IHC (p) WB FITC Mouse IgG1 Log in to see RV202 5
0.0010564557 ABIN1042494 ICC IHC (fro) FACS IHC (p) WB HRP Mouse IgG1 Log in to see RV202 5
0.0010564557 ABIN1805483 ICC IHC (fro) FACS FITC Mouse IgG1 Log in to see RV202
0.0010564557 ABIN4365314 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202 1
0.0010564557 ABIN1875327 FACS IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010564557 ABIN919698 FACS IF (cc) IF (p) Alexa Fluor 350 Rabbit IgG AA 416-466 Log in to see Polyclonal
0.0010564557 ABIN919696 FACS IF (cc) IF (p) Alexa Fluor 488 Rabbit IgG AA 416-466 Log in to see Polyclonal
0.0010564557 ABIN919699 FACS IF (cc) IF (p) Alexa Fluor 555 Rabbit IgG AA 416-466 Log in to see Polyclonal
0.0010564557 ABIN919700 FACS IF (cc) IF (p) Alexa Fluor 647 Rabbit IgG AA 416-466 Log in to see Polyclonal
0.0010564557 ABIN672789 FACS IF (cc) IF (p) Cy3 Rabbit IgG AA 416-466 Log in to see Polyclonal
0.0010564557 ABIN672790 FACS IF (cc) IF (p) Cy5 Rabbit IgG AA 416-466 Log in to see Polyclonal
0.0010564557 ABIN672791 FACS IF (cc) IF (p) Cy5.5 Rabbit IgG AA 416-466 Log in to see Polyclonal
0.0010564557 ABIN672792 FACS IF (cc) IF (p) Cy7 Rabbit IgG AA 416-466 Log in to see Polyclonal

Top referenced anti-Vimentin antibody for Flow Cytometry

Similar anti-Vimentin Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 35 Antibodies 34 Antibodies
Flow Cytometry (FACS) 85 Antibodies 53 Antibodies
Immunochromatography (IC) 3 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 125 Antibodies 97 Antibodies
Immunofluorescence (IF) 150 Antibodies 124 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 12 Antibodies 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 25 Antibodies
Immunohistochemistry (IHC) 106 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 49 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 111 Antibodies
Immunoprecipitation (IP) 31 Antibodies
Simple Western (SimWes) 4 Antibodies
Western Blotting (WB) 228 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Epitope AA 400-500
(23), (17), (16), (16), (16), (15), (14), (13), (12), (8), (7), (7), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus), Mouse (Murine), Human
(806), (293), (253), (205), (118), (110), (95), (77), (75), (42), (39), (32), (30), (29), (25), (7), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(519), (277), (30), (15), (12), (7), (5)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(27), (26), (23), (18), (14), (13), (12), (11), (11), (11), (11), (11), (11), (11), (11), (9), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(671), (299), (290), (290), (239), (233), (231), (128), (104), (25), (15), (12), (8), (8), (5), (3), (3), (3), (3), (1), (1), (1)
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling Images
Specificity Vimentin Antibody, pAb, Rabbit detects endogenous levels of human vimentin protein. Sequence homology predicts that it will also react with mouse and rat vimentin.
Cross-Reactivity (Details) Vimentin Antibody, pAb, Rabbit detects endogenous levels of human vimentin protein. Sequence homology predicts that it will also react with mouse and rat vimentin.
Purification Immunoaffinity chromatography
Immunogen Synthetic peptide (KLH-coupled) derived from within AA 400-500 of human vimentin
Isotype IgG

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin is a member of the intermediate filament protein family. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Despite the fact that most intermediate filaments exist as stable structures, in fibroblasts, vimentin take on a dynamic structure.Vimentin Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 400-500 of human vimentin protein (Swiss Prot: P08670).
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling Images back to top
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLImmunohistochemistry: 5-10 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user-optimized
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Images back to top
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (AA 400-500) (ABIN1574034) Western blot analysis of cell lysates using 1 µg/mL Rabbit Anti-Vimentin Polyclonal A...
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (AA 400-500) (ABIN1574034) Immunohistochemistry analysis of human spleen tissue slide (Paraffin embedded) using ...
Flow Cytometry (FACS) image for anti-Vimentin antibody (VIM) (AA 400-500) (ABIN1574034) Flow cytometric analysis of Ramos cells using Vimentin Antibody, pAb, Rabbit (ABIN398...