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anti-Pig (Porcine) Vimentin antibody for Immunoprecipitation

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Reactivity
Human, Pig (Porcine), Mouse (Murine), Rat (Rattus), Chicken
576
165
147
72
53
41
26
25
15
11
9
9
8
6
5
4
4
3
2
2
1
1
1
1
1
1
1
Host
Mouse
310
261
30
12
11
6
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
23
21
13
10
9
5
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
477
238
185
141
123
85
84
52
46
23
15
11
8
8
3
3
3
3
1
1
1
Supplier
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Catalog No. ABIN94492
$ 265.65
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089396554 ABIN1502034 IP IHC Mouse IgG1 Log in to see VMT-24
0.00089396554 ABIN120783 IF IHC (p) IP WB Mouse IgM Log in to see VI-10
0.00089396554 ABIN1502035 IP Mouse IgG1 Log in to see VMT-24
0.00089396554 ABIN2469991 ICC IP IHC WB Mouse IgM Log in to see VI-10
0.00089396554 ABIN1724173 ICC IHC (p) IP WB Mouse IgM Log in to see VI-10
0.00089396554 ABIN378788 IP WB Mouse IgG1, kappa Log in to see V9
0.00089396554 ABIN269191 ICC IF IHC (p) IP IHC WB Mouse IgM Log in to see VI-10
0.00089396554 ABIN355074 ICC IP ELISA WB Mouse IgM Log in to see
0.00089396554 ABIN378787 IP WB Mouse IgG1, kappa Log in to see V9
0.00089396554 ABIN3200820 ICC IHC (p) IP WB Mouse IgM Log in to see VI-10
0.00089396554 ABIN3040253 ICC IF IP IHC WB Mouse IgM Log in to see

Top referenced anti-Vimentin antibody for Immunoprecipitation

Similar anti-Vimentin Antibodies

Application / Reactivity Human Pig (Porcine) Mouse (Murine) Rat (Rattus) Chicken
Western Blotting (WB) 449 Antibodies 47 Antibodies 125 Antibodies 134 Antibodies 33 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Luminex Assay (LMNX) 8 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Immunoprecipitation (IP) 42 Antibodies 12 Antibodies 8 Antibodies 16 Antibodies 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 133 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human, Pig (Porcine), Mouse (Murine), Rat (Rattus), Chicken
(576), (165), (147), (72), (53), (41), (26), (25), (15), (11), (9), (9), (8), (6), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(310), (261), (30), (12), (11), (6), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(23), (21), (13), (10), (9), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(477), (238), (185), (141), (123), (85), (84), (52), (46), (23), (15), (11), (8), (8), (3), (3), (3), (3), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN94492) Images
Specificity The antibody VI-10 reacts with vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types.
Purification Purified from ascites by precipitation methods and size-exclusion chromatography.
Purity > 95 % (by SDS-PAGE)
Clone VI-10
Isotype IgM

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN94492) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP).Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin.Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN94492) Images back to top
Application Notes Immunohistochemistry (paraffin sections) Recommended dilution: 5 µg/mL
Positive tissue: skin fibroblastImmunocytochemistry
Staining technique: RBL basophilic leukemia cell line: (a) Fix cells for 10 min in methanol at -20 °C and for 6 min in acetone at -20 °C, (b) Fix cells directly in methanol for 10 min at -20 °C or in acetone for 10 min at -20 °C.
Incubation: 45 min RT
Positive control: 3T3 mouse Swiss albino fibroblast cell lineRBL rat basophilic cell line

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN94492) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-Vimentin Antibody (ABIN94492)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Dráberová, Zíková, Dráber: "Monoclonal antibody VI-10 specific for vimentin." in: Folia biologica, Vol. 45, Issue 1, pp. 35-6, 2000

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN94492) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (ABIN94492) Immunofluorescence staining (chicken erythrocytes)
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (ABIN94492) Immunofluorescence staining (rat basophils)
Immunoprecipitation (IP) image for anti-Vimentin antibody (VIM) (ABIN94492) Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10