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anti-Primate Vimentin antibody for Immunohistochemistry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Epitope
AA 2-466
23
16
16
15
14
14
13
12
12
8
7
4
4
4
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Primate
797
291
244
207
121
118
103
79
78
43
40
32
30
27
26
7
5
4
3
2
2
1
1
1
1
1
1
Host
Mouse
522
268
30
12
12
7
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
28
26
21
18
13
13
11
11
11
11
11
11
11
11
11
11
9
5
5
5
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
666
293
290
288
240
227
219
131
104
23
15
11
8
8
5
3
3
3
3
1
1
1
Supplier
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Catalog No. ABIN2869381
€ 442.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011325554 ABIN4365314 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202
0.0011325554 ABIN4365462 FACS ICC IF IHC IHC (fro) IHC (p) FITC Mouse IgG1 Log in to see RV202
0.0011325554 ABIN4365303 ELISA IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 4F2E9
0.0011325554 ABIN4365304 ELISA IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 4F2E9
0.0011325554 ABIN4365315 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202
0.0011325554 ABIN4365383 ICC IF IHC IHC (p) Mouse IgM Log in to see 43BE8

Top referenced anti-Vimentin antibody for Immunohistochemistry

Similar anti-Vimentin Antibodies

Application / Reactivity Primate Human
Immunohistochemistry (IHC) 7 Antibodies 280 Antibodies
Western Blotting (WB) 5 Antibodies
ELISA 211 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 217 Antibodies
Immunochromatography (IC) 12 Antibodies
Immunocytochemistry (ICC) 240 Antibodies
Immunodiffusion (ID) 3 Antibodies
Immunoelectrophoresis (IEP) 3 Antibodies
Immunofluorescence (IF) 283 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 119 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 281 Antibodies
Immunoprecipitation (IP) 99 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Epitope AA 2-466
(23), (16), (16), (15), (14), (14), (13), (12), (12), (8), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Primate
(797), (291), (244), (207), (121), (118), (103), (79), (78), (43), (40), (32), (30), (27), (26), (7), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(522), (268), (30), (12), (12), (7), (5)
Clonality
Monoclonal
Conjugate This Vimentin antibody is un-conjugated
(28), (26), (21), (18), (13), (13), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (9), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(666), (293), (290), (288), (240), (227), (219), (131), (104), (23), (15), (11), (8), (8), (5), (3), (3), (3), (3), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN2869381) Images
Specificity Purified recombinant fragment of Vimentin(aa2-466) expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen Purified recombinant fragment of Vimentin(aa2-466) expressed in E. Coli.
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN2869381) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 53652 DA
Gene ID 12406
NCBI Accession NP_003371
UniProt P08670
Research Area Phospho-specific antibodies, Cell Structure
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN2869381) Images back to top
Application Notes Western Bloting: = 1:500 - 2000, Immunohistochemistry: = 1:200 - 1000.
Comment

Background: Vimentin, also know as VIM. It is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN2869381) Images back to top
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-Vimentin Antibody (ABIN2869381)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Yates, Zetterberg, Rajeev, Reiss, Rittling: "Promoter-independent regulation of vimentin expression in mammary epithelial cells by val(12)ras and TGFbeta." in: Experimental cell research, Vol. 313, Issue 17, pp. 3718-28, 2007

Gilles, Polette, Mestdagt, Nawrocki-Raby, Ruggeri, Birembaut, Foidart: "Transactivation of vimentin by beta-catenin in human breast cancer cells." in: Cancer research, Vol. 63, Issue 10, pp. 2658-64, 2003