anti-Rat (Rattus) Vimentin antibody for Western Blotting

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Epitope
pSer55
23
22
18
17
17
16
16
15
14
9
7
7
6
6
6
5
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
856
366
316
220
138
132
118
83
81
52
50
43
41
37
30
12
8
6
5
5
5
3
3
2
2
1
1
1
1
1
Host
Mouse
523
300
29
14
12
9
8
6
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
28
27
24
21
15
14
12
11
11
11
11
11
11
11
11
10
5
5
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
678
292
289
269
241
227
219
114
84
40
15
14
13
10
8
8
5
3
3
3
1
1
1
1
1
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Catalog No. ABIN1109485
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1873004 ABIN1449160 IF WB Rat IgG2a pSer50 Log in to see TM50 7
3.1873004 ABIN2773915 IHC WB Rabbit N-Term Log in to see Polyclonal 1
3.1873004 ABIN2783289 IHC WB Rabbit C-Term Log in to see Polyclonal 1
3.1873004 ABIN1109486 IF WB Rat IgG2a Log in to see TM71 9
3.1873004 ABIN1109487 IF WB Mouse IgG2b Log in to see MO82 14
3.1873004 ABIN1533459 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
3.1873004 ABIN268950 ICC IF WB Mouse IgM Log in to see VI-01 5
3.1873004 ABIN223315 WB Rabbit IgG Log in to see Polyclonal 2
3.1873004 ABIN152563 ICC IF IHC IHC (fro) IHC (p) WB Chicken IgY Log in to see Polyclonal 18
3.1873004 ABIN441334 ICC IF IHC IHC (fro) IHC (p) IP WB Rabbit IgG Center Log in to see Polyclonal 2
3.1873004 ABIN3042344 IHC (p) WB Rabbit IgG AA 435-466, C-Term Log in to see Polyclonal
3.1873004 ABIN967290 IF IHC WB Mouse IgG1 Log in to see RV202 4
3.1873004 ABIN672786 FACS IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 416-466 Log in to see Polyclonal 27
3.1873004 ABIN261651 ICC IF IHC (p) IHC WB Mouse IgM Log in to see J144 1
3.1873004 ABIN1531759 IHC ELISA WB Rabbit IgG pSer83 Log in to see Polyclonal
3.1873004 ABIN2854814 ICC IHC (fro) IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.1873004 ABIN3182647 ELISA IHC WB Rabbit IgG pSer83 Log in to see Polyclonal
3.1873004 ABIN3187473 ELISA IF IHC WB Rabbit IgG Tyr319 Log in to see Polyclonal
3.1873004 ABIN1532789 ELISA WB Rabbit IgG Log in to see Polyclonal
3.1873004 ABIN3187471 ELISA IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-Vimentin antibody for Western Blotting

Similar anti-Vimentin Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 13 Antibodies 8 Antibodies 8 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies 5 Antibodies 3 Antibodies
Simple Western (SimWes) 5 Antibodies 5 Antibodies 4 Antibodies
Western Blotting (WB) 648 Antibodies 236 Antibodies 275 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies 1 Antibodies 1 Antibodies
Luminex Assay (LMNX) 8 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Epitope pSer55
(23), (22), (18), (17), (17), (16), (16), (15), (14), (9), (7), (7), (6), (6), (6), (5), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(856), (366), (316), (220), (138), (132), (118), (83), (81), (52), (50), (43), (41), (37), (30), (12), (8), (6), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(523), (300), (29), (14), (12), (9), (8), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(28), (27), (24), (21), (15), (14), (12), (11), (11), (11), (11), (11), (11), (11), (11), (10), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunofluorescence (IF), Western Blotting (WB)
(678), (292), (289), (269), (241), (227), (219), (114), (84), (40), (15), (14), (13), (10), (8), (8), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 11 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling ProductDetails: References for anti-Vimentin antibody (ABIN1109485) Images
Sequence SLYSS-phosphoS55-PGGAYC-KLH
Specificity This antibody reacts specifically with the phosphorylated MPV55 peptide but not the non-phosphorylated peptide. This antibody detects vimentin phosphorylated by cdc2 kinase and does not detect non-phosphorylated vimentin or phosphorylated vimentin by cAMP-dependent kinase, protein kinase C, or Ca2+-calmodulin-dependent protein kinase II on Western blotting.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Protein A agarose
Immunogen Synthetic MPV55 phosphopeptide corresponding to mouse phosphorylated vimentin Ser55.
Clone 4A4
Isotype IgG2b

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling ProductDetails: References for anti-Vimentin antibody (ABIN1109485) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin is an intermediate filament protein distributed widely in the cytoplasm and is phosphorylated by several protein kinase in vitro. Ser55 residues on vimentin were reported to be one of the phosphorylation sites of vimentin at metaphase and were the phosphorylation sites for cdc2 kinase but not for cAMP-dependent protein kinase, protein kinase C, and Ca2+-calmodulin-dependent protein kinase II in vitro. Immunofluorescence and immunoelectron microscopy showed that vimentin Ser55 residues distributed in the entire cytoplasmic vimentin filament system are phosphorylated when the cells enter mitosis and de-phosphorylated in cytokinesis. The use of this antibody that specifically reacts with the phosphorylation site of vimentin Ser55 by cdc2 kinase enables estimation of a particular cdc2 kinase function.Synonyms: VIM
Gene ID 7431
NCBI Accession NP_003371
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling ProductDetails: References for anti-Vimentin antibody (ABIN1109485) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details ProductDetails: References for anti-Vimentin antibody (ABIN1109485) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 50 % glycerol, pH 7.2. No preservative is contained.
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.

ProductDetails: References for anti-Vimentin antibody (ABIN1109485)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Tatsumi, Sugimoto, Yugawa, Narisawa-Saito, Kiyono, Fujita: "Deregulation of Cdt1 induces chromosomal damage without rereplication and leads to chromosomal instability." in: Journal of cell science, Vol. 119, Issue Pt 15, pp. 3128-40, 2006

Oguri, Inoko, Shima, Izawa, Arimura, Yamaguchi, Inagaki, Kaibuchi, Kikuchi, Inagaki: "Vimentin-Ser82 as a memory phosphorylation site in astrocytes." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 5, pp. 531-40, 2006

Shiromizu, Goto, Tomono, Bartek, Totsukawa, Inoko, Nakanishi, Matsumura, Inagaki: "Regulation of mitotic function of Chk1 through phosphorylation at novel sites by cyclin-dependent kinase 1 (Cdk1)." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 5, pp. 477-85, 2006

Kohn, Aladjem, Weinstein, Pommier: "Molecular interaction maps of bioregulatory networks: a general rubric for systems biology." in: Molecular biology of the cell, Vol. 17, Issue 1, pp. 1-13, 2005

Tsai, Chen, Kriegstein, Vallee: "LIS1 RNA interference blocks neural stem cell division, morphogenesis, and motility at multiple stages." in: The Journal of cell biology, Vol. 170, Issue 6, pp. 935-45, 2005

Englund, Fink, Lau, Pham, Daza, Bulfone, Kowalczyk, Hevner: "Pax6, Tbr2, and Tbr1 are expressed sequentially by radial glia, intermediate progenitor cells, and postmitotic neurons in developing neocortex." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 1, pp. 247-51, 2005

Miyata, Kawaguchi, Saito, Kawano, Muto, Ogawa: "Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells." in: Development (Cambridge, England), Vol. 131, Issue 13, pp. 3133-45, 2004

Nakamura, Hashimoto, Amano, Nagata, Matsumoto, Goto, Fukusho, Mori, Kashiwagi, Kudo, Inagaki, Takeda: "Localized phosphorylation of vimentin by rho-kinase in neuroblastoma N2a cells." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 5, Issue 10, pp. 823-37, 2000

Inada, Togashi, Nakamura, Kaibuchi, Nagata, Inagaki: "Balance between activities of Rho kinase and type 1 protein phosphatase modulates turnover of phosphorylation and dynamics of desmin/vimentin filaments." in: The Journal of biological chemistry, Vol. 274, Issue 49, pp. 34932-9, 2000

Goto, Kosako, Tanabe, Yanagida, Sakurai, Amano, Kaibuchi, Inagaki: "Phosphorylation of vimentin by Rho-associated kinase at a unique amino-terminal site that is specifically phosphorylated during cytokinesis." in: The Journal of biological chemistry, Vol. 273, Issue 19, pp. 11728-36, 1998

Scholey: "Kinesin-II, a membrane traffic motor in axons, axonemes, and spindles." in: The Journal of cell biology, Vol. 133, Issue 1, pp. 1-4, 1996

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling ProductDetails: References for anti-Vimentin antibody (ABIN1109485) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (pSer55) (ABIN1109485) anti-Vimentin (VIM) (pSer55) antibody
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (pSer55) (ABIN1109485) anti-Vimentin (VIM) (pSer55) antibody (Image 2)