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anti-Sheep (Ovine) Vimentin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Reactivity
Chicken, Human, Potoroo, Rat (Rattus), Sheep (Ovine)
576
165
148
73
53
41
26
25
15
11
9
9
8
6
4
4
4
2
2
2
1
1
1
1
1
1
1
Host
Mouse
310
261
30
12
11
6
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
23
21
13
10
9
5
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
477
238
185
141
122
85
84
51
47
23
15
11
8
8
3
3
3
3
1
1
1
Supplier
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Catalog No. ABIN112184
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089488085 ABIN2964611 ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see V9
0.00089488085 ABIN3026897 FACS IF IHC (p) Mouse IgM, kappa Log in to see LN-6
0.00089488085 ABIN3026899 FACS IF IHC (p) Mouse IgM, kappa Log in to see LN-6

Top referenced anti-Vimentin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-Vimentin Antibodies

Application / Reactivity Sheep (Ovine) Rat (Rattus)
Flow Cytometry (FACS) 4 Antibodies 17 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 35 Antibodies
Immunofluorescence (IF) 3 Antibodies 52 Antibodies
Immunohistochemistry (IHC) 1 Antibodies 64 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies 46 Antibodies
Western Blotting (WB) 2 Antibodies 134 Antibodies
ELISA 32 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunoprecipitation (IP) 16 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Chicken, Human, Potoroo, Rat (Rattus), Sheep (Ovine)
(576), (165), (148), (73), (53), (41), (26), (25), (15), (11), (9), (9), (8), (6), (4), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(310), (261), (30), (12), (11), (6), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(23), (21), (13), (10), (9), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(477), (238), (185), (141), (122), (85), (84), (51), (47), (23), (15), (11), (8), (8), (3), (3), (3), (3), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN112184) Images
Specificity The antibody clone V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Purification Purified
Immunogen V9 is an antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from Porcine lens.
Clone V9
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN112184) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.Synonyms: VIM
Gene ID 7431
NCBI Accession NP_003371
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN112184) Images back to top
Application Notes This antibody is suitable for Immunoblotting, Immunocytochemistry,Immunohistochemistry on frozen sections and paraffin-embedded tissues and Flowcytometry. Recommended dilutions: Flow cytometry: 1/25-1/200. Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent: 1/25-1/200. For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer ( pH 6.0) is required. Immunoblotting applications: 1/100-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN112184) Images back to top
Concentration 1.0 mg/mL
Buffer PBS with 0.09 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Do not freeze working dilutions.
Storage 4 °C/-20 °C
Storage Comment Store the original vial at 2-8 °C. After reconstitution, aliquot and store stock solution or aliquots thereof at -20 °C.

References for anti-Vimentin Antibody (ABIN112184)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Corver, Koopman, van der Aa, Regensburg, Fleuren, Cornelisse: "Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer." in: Cytometry, Vol. 39, Issue 2, pp. 96-107, 2000

Van Muijen, Ruiter, Warnaar: "Coexpression of intermediate filament polypeptides in human fetal and adult tissues." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 57, Issue 4, pp. 359-69, 1987

Van Muijen, Warnaar, Ponec: "Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis." in: Experimental cell research, Vol. 171, Issue 2, pp. 331-45, 1987

Tölle, Weber, Osborn: "Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple keratins allow insight into the composition of particular 10 nm filaments." in: European journal of cell biology, Vol. 38, Issue 2, pp. 234-44, 1985

Osborn, Debus, Weber: "Monoclonal antibodies specific for vimentin." in: European journal of cell biology, Vol. 34, Issue 1, pp. 137-43, 1984