anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) Antibodies

CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. Additionally we are shipping CDC20 Proteins (7) and and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
CDC20 991 Q12834
CDC20 107995 Q9JJ66
CDC20 64515 Q62623
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Top anti-CDC20 Antibodies at antibodies-online.com

Showing 10 out of 263 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated IHC (p), WB Western blot analysis of Cdc20 using anti-Cdc20 antibody . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.  Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc20 antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc20 at approximately 55KD. The expected band size for Cdc20 is at 55KD. IHC analysis of Cdc20 using anti-Cdc20 antibody .Cdc20 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Cdc20 Antibody  overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg Log in to see 4 to 6 Days
$280.00
Details
Cow Rabbit Un-conjugated IHC, WB WB Suggested Anti-CDC20 Antibody Titration:  0.2-1 ug/ml  ELISA Titer:  1:62500  Positive Control:  Hela cell lysate CDC20 is supported by BioGPS gene expression data to be expressed in HeLa 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated EIA, IP, WB Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked headto- tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below. 0.1 mL Log in to see 6 to 8 Days
$533.50
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), IP, PLA, WB Western Blot and Immunoprecipitation: CDC20 Antibody [ABIN252981] - Detection of Human CDC20 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB, 1 mg for IP, 20% of IP loaded) and 293T (T, 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CDC20 antibody ABIN252981 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CDC20 was also immunoprecipitated by rabbit anti-CDC20 antibody ABIN252980, which recognizes an upstream epitope. Immunohistochemistry-Paraffin: Cdc20 Antibody [ABIN2529818] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:250. 0.1 mL Log in to see 7 to 9 Days
$556.31
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), IP Western Blot: CDC20 Antibody [ABIN252980] - Detection of Human CDC20 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDC20 antibody ABIN252980 used for IP at 3 mcg/mg lysate. CDC20 was also immunoprecipitated by rabbit anti- CDC20 antibody ABIN252981, which recognizes a downstream epitope. Immunohistochemistry-Paraffin: Cdc20 Antibody [ABIN2529807] - Detection of Mouse CDC20 by Immunohistochemistry. FFPE section of mouse teratoma. Affinity purified rabbit anti-CDC20 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit. 100 μL Log in to see 7 to 9 Days
$556.31
Details
Human Rabbit Un-conjugated WB Western blot analysis of CDC20 expression in Raji (A), HEK293T (B), HL60 (C) whole cell lysates. 200 μL Log in to see 13 to 14 Days
$487.50
Details
Human Rabbit Un-conjugated IF, IHC, WB Western blot analysis of extracts of various cell lines, using CDC20 antibody. 100 μL Log in to see 16 Days
$334.77
Details
Human Rabbit Un-conjugated ELISA, WB Western blot analysis of extracts from Jurkat/HeLa cells, using p55CDC Antibody. The lane on the right is treated with the synthesized peptide. 100 μg Log in to see 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated ELISA, IHC, WB Western Blot (WB) analysis of specific cells using Cdc20 Polyclonal Antibody. 100 μL Log in to see 16 Days
$181.73
Details
Bat Rabbit Un-conjugated IHC, IHC (p), WB Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Anti-CDC20 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other for ... 50 μg Log in to see 11 to 14 Days
$727.83
Details

Top referenced anti-CDC20 Antibodies

  1. Human Polyclonal CDC20 Primary Antibody for ICC, IF - ABIN252981 : Di Fiore, Pines: How cyclin A destruction escapes the spindle assembly checkpoint. in The Journal of cell biology 2010 (PubMed)
    Show all 3 Pubmed References

  2. Human Polyclonal CDC20 Primary Antibody for ICC, IF - ABIN252980 : Ahlskog, Björk, Elsing, Aspelin, Kallio, Roos-Mattjus, Sistonen: Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress. in Molecular and cellular biology 2010 (PubMed)
    Show all 2 Pubmed References

  3. Xenopus laevis Monoclonal CDC20 Primary Antibody for CyTOF, ELISA - ABIN251088 : Sackton, Dimova, Zeng, Tian, Zhang, Sackton, Meaders, Pfaff, Sigoillot, Yu, Luo, King: Synergistic blockade of mitotic exit by two chemical inhibitors of the APC/C. in Nature 2014 (PubMed)

  4. Human Polyclonal CDC20 Primary Antibody for IP, WB - ABIN233786 : Yamamuro, Kano, Naito: Functions of FZR1 and CDC20, activators of the anaphase-promoting complex, during meiotic maturation of swine oocytes. in Biology of reproduction 2008 (PubMed)

More Antibodies against CDC20 Interaction Partners

Human Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) interaction partners

  1. we demonstrate that the CDC20-MAD2 (show MAD2L1 Antibodies) complex could also be formed independently of the SAC (show ADCY10 Antibodies). Moreover, in prolonged arrest caused by nocodazole treatment, the overall levels of the CDC20-MAD2 (show MAD2L1 Antibodies) complex are gradually, but significantly, reduced and this is associated with lower levels of cyclin B1 (show CCNB1 Antibodies), which brings a new insight into the mechanism of mitotic "slippage" of the arrested cells.

  2. Cdc20 may be a promising molecular target for chemotherapy.

  3. Long non-coding RNA SPRY4 (show SPRY4 Antibodies)-IT1 (show HAUS3 Antibodies) promotes cell proliferation and invasion by regulation of Cdc20 in pancreatic cancer cells

  4. Cdc20 functions as an important negative regulator of SMAR1 (show BANP Antibodies) in higher grades of cancer

  5. Data provide support for the recent structure-based models and functionally dissect three elements of Cdc20 inhibition: sequestration of Cdc20 in the core mitotic checkpoint (show BUB3 Antibodies) complex, sufficient at low Cdc20 concentrations; inhibition of a second Cdc20 through the Mad3 (show SMAD3 Antibodies) C terminus, independent of Mad2 (show MAD2L1 Antibodies) binding to this Cdc20 molecule; and occupancy of the APC (show APC Antibodies)/C with full MCC (show MCC Antibodies), where Mad3 (show SMAD3 Antibodies) and Apc15 (show ANAPC15 Antibodies) are involved.

  6. CDC20 gene, related to cell proliferation in protein complex A, might play momentous roles in the initiation and development of consecutive Trauma-Induced Sepsis.

  7. A mitotic phosphorylation site on Cdc20, known to be a substrate of PP2A (show PPP2R4 Antibodies)(B56), modulates APC (show APC Antibodies)/C(Cdc20) assembly.

  8. c-Myc (show MYC Antibodies) is a driver when combined with kRas/Akt3 (show AKT3 Antibodies) oncogenic signals in gliomagenesis, whereas Cdc20 overexpression is a passenger

  9. The ABBA (show MTSS1L Antibodies)-KEN (show PCNT Antibodies)-ABBA (show MTSS1L Antibodies) amino acid motif cassette holds the Mitotic Checkpoint (show BUB3 Antibodies) Complex (MCC (show MCC Antibodies)) onto the Anaphase-Promoting Complex-Cyclosome (APC (show APC Antibodies)/C) by binding the two Cdc20 molecules in the MCC (show MCC Antibodies)-APC (show APC Antibodies)/C complex.

  10. It discuses the roles of Cdc20 in SAC (show ADCY10 Antibodies) signalling and mitotic exit, describe how the integration of traditional approaches with emerging technologies has revealed new details of Cdc20 functions, comment about the potential of Cdc20 as a therapeutic target for the treatment of human malignancies.

Xenopus laevis Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) interaction partners

  1. Cdc20 auto-ubiquitylation does not play a major role in terminating Cdc20 activation.

  2. Dephosphorylation of Cdc20 is required for its loading and activation of the APC/C ubiquitin ligase.

  3. A role for the fizzy/cdc20 family of proteins in activation of the APC (show APC Antibodies)/C distinct from substrate recruitment is reported.

Mouse (Murine) Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) interaction partners

  1. Cdc20 hypomorphism causes chromatin bridging and chromosome misalignment, revealing a requirement for Cdc20 in efficient sister chromosome separation and chromosome-microtubule attachment.

  2. The physiologically effective threshold level of Cdc20 is high for female meiosis I.

  3. Results indicate that Cdc20 also contributes to post-anaphase activation of the APC (show APC Antibodies)/C.

  4. The seven tandem WD motifs of Cdc20 they are required for speriolin binding and for localization of Cdc20 to the centrosomes and nucleus, suggesting that speriolin might regulate or stabilize the folding of Cdc20 during meiosis in spermatogenic cells

  5. Data suggest that Mad2 (show MXI1 Antibodies) and BubR1 (show BUB1B Antibodies) must cooperate to inhibit Cdc20 activity.

  6. Cdc20 is degraded through two independent degradation signals (degrons), the KEN (show PCNT Antibodies) box and a newly described CRY (show CRY2 Antibodies) box.

  7. Cdc20 and securin (show PTTG1 Antibodies) double mutant embryos could not maintain the metaphase arrest, suggesting a role of securin (show PTTG1 Antibodies) in preventing mitotic exit

  8. Expression of the cdc20 gene is down-regulated by zif268 (show EGR1 Antibodies) in neuronal cells; altered expression of proteasome-regulatory genes following zif268 (show EGR1 Antibodies) induction may be a key component of long-lasting CNS plasticity.

  9. Cdc20 is required for the anaphase onset of the first meiosis but not the second meiosis in mouse oocytes

  10. findings suggest a novel function of HSF1 (show HSF1 Antibodies) frequently overexpressed in cancer cells, to inhibit APC (show APC Antibodies)/C activity by interacting with Cdc20, and to result in aneuploidy development and genomic instability

Pig (Porcine) Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) interaction partners

  1. porcine FZR1 (show FZR1 Antibodies) and CDC20 work on the maintenance of meiotic arrest at the first meiotic prophase and on the exit from M1

CDC20 Antigen Profile

Protein Summary

CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation.

Gene names and symbols associated with CDC20

  • cell division cycle 20 (cdc20) antibody
  • cell division cycle 20 (CDC20) antibody
  • cell division cycle 20 L homeolog (cdc20.L) antibody
  • cell division cycle 20 (Cdc20) antibody
  • cell division cycle 20 homolog (S. cerevisiae) (CDC20) antibody
  • 2310042N09Rik antibody
  • bA276H19.3 antibody
  • C87100 antibody
  • CDC20A antibody
  • fizzy1 antibody
  • p55CDC antibody
  • X-FZY antibody

Protein level used designations for CDC20

cell division cycle 20 homolog , CDC20 cell division cycle 20 homolog , cell division cycle protein 20 homolog , fizzy1 , mmCdc20 , cell cycle protein p55CDC , p55CDC

GENE ID SPECIES
394542 Xenopus (Silurana) tropicalis
991 Homo sapiens
398044 Xenopus laevis
107995 Mus musculus
64515 Rattus norvegicus
424563 Gallus gallus
482534 Canis lupus familiaris
397379 Sus scrofa
515376 Bos taurus
Selected quality suppliers for anti-CDC20 (CDC20) Antibodies
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