anti-Discs, Large Homolog 3 (Drosophila) (DLG3) Antibodies

DLG3 encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Additionally we are shipping DLG3 Proteins (6) and DLG3 Kits (2) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
DLG3 1741 Q92796
DLG3 53310 P70175
DLG3 58948 Q62936
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Top anti-DLG3 Antibodies at antibodies-online.com

Showing 10 out of 119 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-DLG3 Antibody Titration:  0.2-1 ug/ml  ELISA Titer:  1:312500  Positive Control:  Human heart 100 μL Log in to see 2 to 3 Days
$289.00
Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-DLG3 Antibody Titration:  0.2-1 ug/ml  ELISA Titer:  1:1562500  Positive Control:  293T cell lysate DLG3 is supported by BioGPS gene expression data to be expressed in HEK293T 100 μL Log in to see 2 to 3 Days
$289.00
Details
Mammalian Mouse Un-conjugated ISt, IHC, WB   5 mL Log in to see 2 to 6 Days
$394.53
Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-Dlg3 Antibody   Titration: 1.0 ug/ml   Positive Control: Rat Brain 100 μL Log in to see 2 to 3 Days
$289.00
Details
Mouse Rabbit Un-conjugated IHC (p), WB Western blot analysis of SAP102 using anti-SAP102 antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate.  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAP102 antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAP102 at approximately 102KD. The expected band size for SAP102 is at 90KD. IHC analysis of SAP102 using anti-SAP102 antibody . SAP102 was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SAP102 Antibody  overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg Log in to see 4 to 6 Days
$280.00
Details
Human Mouse Un-conjugated AA, ICC, IF, IHC, IP, WB Rat brain extract copy. 100 μg Log in to see 3 to 4 Days
$374.40
Details
Human Mouse FITC AA, ICC, IF, IHC, IP, WB Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SAP102 Antibody (D) Composite. Western Blot analysis of Rat brain lysates showing detection of SAP102 protein using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 . Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 100 μg Log in to see 3 to 4 Days
$425.10
Details
Human Mouse PerCP AA, ICC, IF, IHC, IP, WB Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SAP102 Antibody (D) Composite. Western Blot analysis of Rat brain lysates showing detection of SAP102 protein using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 . Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 100 μg Log in to see 3 to 4 Days
$434.20
Details
Human Mouse APC AA, ICC, IF, IHC, IP, WB Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SAP102 Antibody (D) Composite. Western Blot analysis of Rat brain lysates showing detection of SAP102 protein using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 . Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 100 μg Log in to see 3 to 4 Days
$434.20
Details
Human Mouse Atto 633 AA, ICC, IF, IHC, IP, WB Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SAP102 Antibody (D) Composite. Western Blot analysis of Rat brain lysates showing detection of SAP102 protein using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 . Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 100 μg Log in to see 3 to 4 Days
$435.50
Details

Top referenced anti-DLG3 Antibodies

  1. Mammalian Monoclonal DLG3 Primary Antibody for ISt, IHC - ABIN1304941 : Williams, Featherstone: Regulation of hippocampal synaptic strength by glial xCT. in The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 (PubMed)
    Show all 27 Pubmed References

  2. Human Polyclonal DLG3 Primary Antibody for ICC, WB - ABIN1742274 : Ludford-Menting, Oliaro, Sacirbegovic, Cheah, Pedersen, Thomas, Pasam, Iazzolino, Dow, Waterhouse, Murphy, Ellis, Smyth, Kershaw, Darcy, Humbert, Russell: A network of PDZ-containing proteins regulates T cell polarity and morphology during migration and immunological synapse formation. in Immunity 2005 (PubMed)
    Show all 6 Pubmed References

  3. Mouse (Murine) Polyclonal DLG3 Primary Antibody for ICC, IP - ABIN2690526 : Fujita-Jimbo, Tanabe, Yu, Kojima, Mori, Li, Iwamoto, Yamagata, Momoi, Momoi: The association of GPR85 with PSD-95-neuroligin complex and autism spectrum disorder: a molecular analysis. in Molecular autism 2015 (PubMed)

  4. Cow (Bovine) Polyclonal DLG3 Primary Antibody for WB - ABIN2781528 : Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: Large-scale mapping of human protein-protein interactions by mass spectrometry. ... in Molecular systems biology 2007 (PubMed)

  5. Rat (Rattus) Monoclonal DLG3 Primary Antibody for ICC, IF - ABIN447355 : Lecointre, Vézier, Bénard, Ramdani, Dupré, Brasse-Lagnel, Henry, Roy, Marret, Gonzalez, Jégou, Leroux-Nicollet: Age-dependent alterations of the NMDA receptor developmental profile and adult behavior in postnatally ketamine-treated mice. in Developmental neurobiology 2015 (PubMed)

  6. Human Polyclonal DLG3 Primary Antibody for ICC, IF - ABIN4351980 : Qu, Aronica, Boer, Fällmar, Kumlien, Nistér, Wester, Pontén, Smits: DLG3/SAP102 protein expression in malformations of cortical development: a study of human epileptic cortex by tissue microarray. in Epilepsy research 2009 (PubMed)

More Antibodies against DLG3 Interaction Partners

Human Discs, Large Homolog 3 (Drosophila) (DLG3) interaction partners

  1. These data provide evidence for a novel mechanism in regulating SAP102 function and glutamate receptor trafficking.

  2. This family broadens the mutational and phenotypical spectrum of DLG3-associated non-syndromic X-linked intellectual disability and demonstrates that heterozygous female mutation carriers can be as severely affected as males.

  3. Following the critical period NMDA receptor function was unaffected by loss of SAP102 but there was a reduction in the divergence of TC connectivity. These data suggest that changes in synaptic function early in development caused by mutations in SAP102 result in changes in network connectivity later in life.

  4. Insertion of a guanine into the DLG3 5' UTR, 7 bp upstream of the start codon, down regulated DLG3 protein levels. This non-coding variant segregates with X-linked intellectual disability in a large family.

  5. Trans-homophilic interaction of CADM1 activates PI3K by forming a complex with MAGuK-family proteins MPP3 and Dlg.

  6. miR-1246 might play a role in neurological pathogenesis of human enterovirus 71 by regulating DLG3 gene in infected cells.

  7. These data shed new light on the role of SAP102 in the regulation of NMDAR trafficking.

  8. This study identified DLG3 significantly associated loci with a biologically plausible role in schizophrenia.

  9. The data of this study suggested that DLG3 is down-regulated in this cancer type.

  10. The PDZ-independent interaction between SAP102 and GluN2B mediates the synaptic clearance of GluN2B-containing NMDARs.(SAP102 protein)

  11. Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin.

  12. A total of six novel and 11 known single nucleotide polymorphisms were identified. Further studies are warranted to analyze the candidate genes at Xq11.1-q21.33.

  13. DLG3 was identified by genome-wide gene expression analyses as correlated with cellular sensitivity to cisplatin and carboplatin. DLG3 was also found to correlate with cellular sensitivity to platinating agents in NCI-60 cancer cell lines.

  14. DLG3 did not associate with non-syndromic mental retardation in Chinese Han population; however, further studies are needed.

  15. Results identified a novel splice site mutation in the disc-large homolog 3 (DLG3) gene, encoding the synapse-associated protein 102 (SAP102) in one out of 300 families with moderate to severe non-syndromic mental retardation.

  16. Loss may lead to altered synaptic plasticity and may explain the intellectual impairment observed in individuals with DLG3 mutations

  17. E6AP is extensively involved in the ubiquitin-mediated degradation of Dlg (an HPV E6-dependent substrate) as a cellular E3 ubiquitin-protein ligase.

  18. The results of this study suggested a putative role for DLG3/SAP102 in cortical hyperexcitability and epileptogenicity of malformations of cortical development.

Mouse (Murine) Discs, Large Homolog 3 (Drosophila) (DLG3) interaction partners

  1. Following the critical period NMDA receptor function was unaffected by loss of SAP102 but there was a reduction in the divergence of TC connectivity. These data suggest that changes in synaptic function early in development caused by mutations in SAP102 result in changes in network connectivity later in life.

  2. our current study identified a functional interplay between PSD-95 and synapse-associated protein 102 (SAP102) to regulate synaptic AMPA receptors

  3. SAP102 has a fundamental role in trafficking the NMDA receptor complex to the synaptic sites.

  4. The Dlg3 have functions in complex cognitive processes.

  5. Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin.

  6. Dlg3 exerts a distinct function by recruiting the ubiquitin ligases Nedd4 and Nedd4-2 through its PPxY motifs. These interactions are required for Dlg3 monoubiquitination, apical membrane recruitment, and tight junction consolidation.

  7. Membrane-associated guanylate kinase homolog SAP102 is a central organizers of the postsynaptic density at excitatory synapses on pyramidal neurons.

  8. SAP102 interacts with the PDZ-binding domain of Sec8, a member of the exocyst complex. nteractions between the two proteins are involved in the delivery of N-methyl-D-aspartate receptors to the cell surface in heterologous cells and neurons.

  9. SAP102 was required for normal spatial learning. Loss of SAP102 results in changes in spatial strategy usage. Altered long term potentiation and spike-timing-dependent plasticity in SAP102 mutants. Couples NMDA receptors to MAPK/ERK pathway.

  10. Results describe E-dlg, a mouse homologue of the Drosophila discs large tumor suppressor (Dlg, also known as SAP97), that binds preferentially to SAP102.

  11. SAP102 and PSD-95 regulate the synaptic trafficking of distinct glutamate receptor subtypes at different developmental stages, thereby playing necessary roles in excitatory synapse development.

DLG3 Antigen Profile

Protein Summary

This gene encodes a member of the membrane-associated guanylate kinase protein family. The encoded protein may play a role in clustering of NMDA receptors at excitatory synapses. It may also negatively regulate cell proliferation through interaction with the C-terminal region of the adenomatosis polyposis coli tumor suppressor protein. Mutations in this gene have been associated with X-linked mental retardation. Alternatively spliced transcript variants have been described.

Gene names and symbols associated with DLG3

  • discs large MAGUK scaffold protein 3 (DLG3) antibody
  • discs, large homolog 3 (Drosophila) (dlg3) antibody
  • discs, large homolog 3 (Drosophila) (Dlg3) antibody
  • discs large MAGUK scaffold protein 3 (Dlg3) antibody
  • Dlgh3 antibody
  • fa66c08 antibody
  • fa66e02 antibody
  • fd02c12 antibody
  • fu95c12 antibody
  • im:7138640 antibody
  • mKIAA1232 antibody
  • MPP3 antibody
  • MRX antibody
  • MRX90 antibody
  • NEDLG antibody
  • SAP102 antibody
  • si:ch211-276g21.1 antibody
  • wu:fa66c08 antibody
  • wu:fa66e02 antibody
  • wu:fd02c12 antibody
  • wu:fu95c12 antibody
  • XLMR antibody

Protein level used designations for DLG3

disks large homolog 3 , neuroendocrine-DLG , synapse-associated protein 102 , SAP-102 , discs large homolog 3 , discs, large homolog 3 (neuroendocrine-dlg, Drosophila) , membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3) , PSD-95/SAP90-related protein 1 , SAP102

GENE ID SPECIES
1741 Homo sapiens
564081 Danio rerio
53310 Mus musculus
428706 Gallus gallus
491942 Canis lupus familiaris
100516124 Sus scrofa
509882 Bos taurus
58948 Rattus norvegicus
100732206 Cavia porcellus
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