RFP Antibodies
(Red Fluorescent Protein (RFP))
Antibodies that detect Red Fluorescent Protein (RFP) are used in various biological and biomedical research applications (e.g. Western Blot) to visualize and study cellular processes, protein localization, and gene expression. Red Fluorescent Protein is a type of fluorescent protein that emits red light when exposed to specific wavelengths of light. It's often used as a molecular tag to label proteins and other cellular structures in live or fixed cells and tissues.
Here are some common applications of RFP antibodies:
- Cellular Localization and Protein Trafficking: Researchers can fuse the RFP protein to their protein of interest. By using an RFP antibody, they can detect the presence and subcellular localization of the fusion protein within cells. This helps in understanding the dynamics and movement of proteins within cellular compartments.
- Gene Expression Studies: RFP can also be used as a marker for gene expression. Researchers can use RFP-tagged constructs to monitor the expression of specific genes. Antibodies against RFP are then used to detect the RFP-tagged protein produced from these genes.
- Protein Interaction Studies: RFP can be used in protein-protein interaction studies. Proteins of interest are tagged with RFP and their interactions with other proteins are investigated. Antibodies against RFP can then be used to detect these interactions either through immunoprecipitation or other methods.
- Live Cell Imaging: RFP-tagged proteins can be imaged in real-time within live cells using fluorescence microscopy. This allows researchers to track protein dynamics, localization changes, and cellular responses in real-time.
- Flow Cytometry: Antibodies against RFP can be used in flow cytometry (FACS) to quantify the expression levels of RFP-tagged proteins in a population of cells. This is particularly useful for high-throughput studies.
- High-Content Screening: RFP antibodies can be used in high-content screening assays to study various cellular processes and responses across large sets of conditions or compounds.
- Visualization of Cellular Structures: RFP can be fused to specific cellular structures such as organelles, cytoskeletal components, or membranes. Antibodies against RFP allow researchers to visualize these structures and their dynamics.
- Co-localization Studies: Antibodies against RFP can be used in combination with antibodies against other fluorescent proteins to study co-localization and potential interactions between different cellular components.
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54 results
Independently Validated
Rockland Original
RFP
Reactivity: Discosoma
WB, IF, IHC (fro), IHC (p)
Host: Goat
Polyclonal
unconjugated
2 references
Rockland 600-901-379
RFP
Reactivity: Discosoma
WB, ELISA
Host: Chicken
Polyclonal
unconjugated
27 references
Rockland Original
RFP
Reactivity: Discosoma
WB, IF, IHC (fro)
Host: Goat
Polyclonal
DyLight 550
RFP
Reactivity: Discosoma
WB, IF, IHC (fro)
Host: Goat
Polyclonal
DyLight 633
RFP
Reactivity: Discosoma
WB, IF, IHC (fro)
Host: Goat
Polyclonal
DyLight 488
Rockland 600-406-379
RFP
Reactivity: Discosoma
WB, ELISA, IHC
Host: Rabbit
Polyclonal
Biotin
4 references
Rockland Original
Rockland 600-906-379
RFP
Reactivity: Discosoma
WB, ELISA, IF, FM
Host: Chicken
Polyclonal
Biotin
1 reference
Rockland Original
RFP
Reactivity: Discosoma
WB, IF, IHC (fro)
Host: Goat
Polyclonal
DyLight 405
1 image
RFP
Reactivity: Discosoma
WB, ELISA, IF, IHC, IP, DB
Host: Mouse
Monoclonal
RF5R
unconjugated
1 image
RFP
Reactivity: Discosoma
WB, ELISA
Host: Mouse
Monoclonal
unconjugated
1 image
Rockland 600-403-379
RFP
Reactivity: Discosoma
WB, ELISA, IHC
Host: Rabbit
Polyclonal
HRP
1 image
1 reference
Rockland Original
RFP
Reactivity: Discosoma
IP
Host: Mouse
Monoclonal
3G5
Agarose Beads
RFP
Reactivity: Discosoma
IF, FACS, ICC
Host: Alpaca
Monoclonal
2B12
unconjugated
single-domain Antibody (sdAb)
Recombinant Antibody
RFP
Reactivity: Discosoma
WB, ELISA, IF, IHC, IP, DB
Host: Mouse
Monoclonal
RF5R
unconjugated
RFP
Reactivity: Discosoma
IF, FACS, ICC
Host: Alpaca
Monoclonal
2B12-2A1
Alexa Fluor 647
single-domain Antibody (sdAb)
Recombinant Antibody
RFP
Reactivity: Discosoma
IF, ICC
Host: Alpaca
Monoclonal
2B12-2A1
AZDye 568
single-domain Antibody (sdAb)
Recombinant Antibody
RFP
Reactivity: Discosoma
IF, FACS, ICC
Host: Alpaca
Monoclonal
2B12
Alexa Fluor 647
single-domain Antibody (sdAb)
Recombinant Antibody
Latest Publications for our RFP Antibodies
: "Injury primes mutation-bearing astrocytes for dedifferentiation in later life." in: Current biology : CB, (2023) (PubMed).: "Diet suppresses glioblastoma initiation in mice by maintaining quiescence of mutation-bearing neural stem cells." in: Developmental cell, (2023) (PubMed).
: "Molecular sensing of mechano- and ligand-dependent adhesion GPCR dissociation." in: Nature, Vol. 615, Issue 7954, pp. 945-953, (2023) (PubMed).
: "Overexpression of Lin28A in neural progenitor cells in vivo does not lead to brain tumor formation but results in reduced spine density." in: Acta neuropathologica communications, Vol. 9, Issue 1, pp. 185, (2022) (PubMed).
: "A versatile viral toolkit for functional discovery in the nervous system. ..." in: Cell reports methods, Vol. 2, Issue 6, pp. 100225, (2022) (PubMed).
: "Axon guidance at the spinal cord midline-A live imaging perspective." in: The Journal of comparative neurology, (2021) (PubMed).
: "Gene regulatory networks controlling differentiation, survival, and diversification of hypothalamic Lhx6-expressing GABAergic neurons." in: Communications biology, Vol. 4, Issue 1, pp. 95, (2021) (PubMed).
: "Extrinsic Regulators of mRNA Translation in Developing Brain: Story of WNTs." in: Cells, Vol. 10, Issue 2, (2021) (PubMed).
: "Co-activation of Sonic hedgehog and Wnt signaling in murine retinal precursor cells drives ocular lesions with features of intraocular medulloepithelioma." in: Oncogenesis, Vol. 10, Issue 11, pp. 78, (2021) (PubMed).
: "The white matter is a pro-differentiative niche for glioblastoma." in: Nature communications, Vol. 12, Issue 1, pp. 2184, (2021) (PubMed).
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