ABCC8 antibody (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8) (AA 1548-1582)

Details for Product anti-ABCC8 Antibody No. ABIN1027723, Supplier: Log in to see
Antigen
  • ABC36
  • HHF1
  • HI
  • HRINS
  • MRP8
  • PHHI
  • SUR
  • SUR1
  • SUR1delta2
  • TNDM2
  • D930031B21Rik
  • Sur
  • Sur1
  • ATP binding cassette subfamily C member 8
  • ATP-binding cassette, sub-family C (CFTR/MRP), member 8
  • ABCC8
  • Abcc8
Alternatives
anti-Human ABCC8 antibody for ELISA
Epitope
AA 1548-1582, C-Term
71
66
14
4
4
3
3
2
1
1
1
1
1
1
Reactivity
Hamster, Human, Mouse (Murine), Rat (Rattus)
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98
85
70
4
3
2
2
2
1
1
1
1
Host
Mouse
95
29
17
Clonality (Clone)
Monoclonal ()
Conjugate
This ABCC8 antibody is un-conjugated
9
9
7
6
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4
4
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
128
93
64
44
36
21
9
3
2
2
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Fusion protein amino acids 1548-1582 (cytoplasmic C-terminus) of rat SUR1
Clone S289-16
Isotype IgG1
Specificity Detects ~160 kDa. Does not cross-react with SUR2B.
Purification Protein G Purified
Plasmids, Primers & others Plasmids, Primers & others ABCC8 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name SUR1 (ABCC8 Antibody Abstract)
Background Sulfonylurea receptors (SUR) are membrane proteins which are the molecular targets of the sulfonylurea class of anti-diabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. More specifically, SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2) (1). The association of four Kir6.x and four SUR subunits form an ion conducting channel commonly referred to as the KATP channel. The primary function of the sulfonylurea receptor is to sense intracellular levels of the nucleotides ATP and ADP and in response facilitate the open or closing its associated Kir6.x potassium channel. Hence the KATP channel monitors the energy balance within the cell (2).
Gene ID 25559
NCBI Accession NP_037171
UniProt Q09429
Pathways Negative Regulation of Hormone Secretion
Application Notes
  • WB (1:1000)
  • IHC (1:1000)
  • ICC/IF (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of SMC-409 was sufficient for detection of SUR1 in 20 μg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8) (AA 1548-1582), (C-Term) antibody (ABIN1027723) Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Anti...
Western Blotting (WB) image for anti-ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8) (AA 1548-1582), (C-Term) antibody (ABIN1027723) Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protei...
Background publications Nichols: "KATP channels as molecular sensors of cellular metabolism." in: Nature, Vol. 440, Issue 7083, pp. 470-6, 2006 (PubMed).

Campbell, Sansom, Ashcroft: "Potassium channel regulation." in: EMBO reports, Vol. 4, Issue 11, pp. 1038-42, 2003 (PubMed).

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