Nuclear Antigen antibody

Catalog No. ABIN103313

Product Details anti-Nuclear Antigen Antibody

Target: Nuclear Antigen (IPO-38)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nuclear Antigen antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Sterility: Sterile filtered

Immunogen: A BALB/c mouse was immunized with spleen cells of a human patient with hairy cell leukemia.
Immunogentype:Native

Clone: IPO-38

Isotype: IgG

Application Details

Application Notes: This antibody is suitable for ELISA,  immunoprecipitation, western blotting and immunohistochemistry (frozen and formalin/paraffin).  The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells (paraformaldehyde fixed, Triton X-100 permeabilized).  An increase of 400% is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemide).  IPO-38 does not block the binding of the Ki-67 antibody.   Tonsilar tissue is typically used as a positive control.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for Nuclear Antigen

Target: Nuclear Antigen (IPO-38)

Alternative Name: IPO-38 (IPO-38 Products)

Background: The specificity of mAb IPO-38 was demonstrated in different human and murine lymphoid and non-lymphoid cell lines. It does not react with mononuclear cells and granulocytes of healthy donors but does react with nuclei of blood cells of patients with different forms of leukemia and with cells from lymph nodes of patients with Hodgkin's disease and non-hodgkin's lymphoma. Also a positive reaction in nuclei of breast and colorectal tumor cells was demonstrated. IPO-38 may be a good marker of cellular proliferation during monitoring of tumor progression.
Synonyms: Non-histone chromosomal protein HMG-14 antibody, High-mobility group nucleosome-binding domain-containing protein 1 antibody, HMGN1 antibody, HMG14 antibody

Gene ID: 15312

NCBI Accession: NP_032277

UniProt: P18608