HTR7 antibody (N-Term)

Catalog No. ABIN1048278

Product Details anti-HTR7 Antibody

Target: HTR7 (5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7))

Binding Specificity: N-Term

Reactivity: Human, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTR7 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Brand: IHC-plus™

Specificity: Human 5HT7 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Marmoset, Dog (100%) Mouse, Rat, Hamster, Panda (95%) Gorilla, Pig (90%) Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from N-terminal extracellular domain of human 5HT7 Receptor. Percent identity with other species by BLAST analysis: Human, Marmoset, Dog (100%), Mouse, Rat, Hamster, Panda (95%), Gorilla, Pig (90%), Horse (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HTR7

Target: HTR7 (5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7))

Alternative Name: HTR7 / 5-HT7 Receptor (HTR7 Products)

Synonyms: 5-HT7 antibody, 5-HT-dro antibody, 5-HT-dro1 antibody, 5-HT7Dro antibody, 5-HT[7]Dro antibody, 5-HT[[7Dro]] antibody, 5-HT[[7]] antibody, 5-HT[[7]]Dro antibody, 5-HT[[dro1]] antibody, 5-HTdro1 antibody, 5HT-7 antibody, 5HT-R1 antibody, 5HT-dro antibody, 5HT-dro1 antibody, 5HT[[7Dro]] antibody, 5htdror antibody, CG12073 antibody, DRO1 antibody, Dm5HTdro1 antibody, Dmel\\CG12073 antibody, 5Ht7 antibody, 5HT7 antibody, htr7-A antibody, 5-hydroxytryptamine receptor 7 antibody, 5-hydroxytryptamine (serotonin) receptor 7 antibody, 5-hydroxytryptamine (serotonin) receptor 7, adenylate cyclase-coupled antibody, 5-hydroxytryptamine (serotonin) receptor 7, adenylate cyclase-coupled L homeolog antibody, HTR7 antibody, 5-HT7 antibody, LOC752003 antibody, Htr7 antibody, htr7.L antibody

Background: Name/Gene ID: HTR7
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR7, 5-HT7, 5-HT7 receptor, 5-HT-7, 5-hydroxytryptamine 7 receptor, 5HT7, 5-HT-X, 5-hydroxytryptamine receptor 7, 5HT7 Receptor, HT7, Serotonin 5-HT7 receptor, Serotonin receptor 7

Gene ID: 3363

Pathways: JAK-STAT Signaling