Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

ENPP3 antibody (Internal Region)

ENPP3 Reactivity: Human, Dog, Monkey IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048561
  • Target See all ENPP3 Antibodies
    ENPP3 (Ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3))
    Binding Specificity
    • 16
    • 13
    • 8
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 66
    • 34
    • 18
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    Human, Dog, Monkey
    Host
    • 51
    • 15
    Rabbit
    Clonality
    • 52
    • 14
    Polyclonal
    Conjugate
    • 22
    • 7
    • 5
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ENPP3 antibody is un-conjugated
    Application
    • 29
    • 25
    • 25
    • 13
    • 13
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human ENPP3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Dog (100%) Bovine, Horse, Pig (95%) Panda, Bat (84%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 19 amino acid peptide from internal region of human ENPP3. Percent identity with other species by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Dog (100%), Bovine, Horse, Pig (95%), Panda, Bat (84%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product ENPP3 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (15 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    ENPP3 (Ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3))
    Alternative Name
    ENPP3 / CD203c (ENPP3 Products)
    Synonyms
    B10 antibody, CD203c antibody, NPP3 antibody, PD-IBETA antibody, PDNP3 antibody, Npp3 antibody, RB13-6 antibody, AI876438 antibody, ENPP3 antibody, ectonucleotide pyrophosphatase/phosphodiesterase 3 antibody, ectonucleotide pyrophosphatase/phosphodiesterase 3 L homeolog antibody, ENPP3 antibody, Enpp3 antibody, enpp3 antibody, enpp3.L antibody
    Background
    Name/Gene ID: ENPP3
    Family: Phosphodiesterase - Other

    Synonyms: ENPP3, CD203c antigen, CD203c, E-NPP 3, NPP3, Phosphodiesterase-I beta, PD-IBETA, PDNP3, Phosphodiesterase I beta, B10, gp130RB13-6
    Gene ID
    5169
    Pathways
    Regulation of Muscle Cell Differentiation, Negative Regulation of Transporter Activity
You are here:
Support