SLC44A2 antibody (Internal Region)

Catalog No. ABIN1049339

Product Details anti-SLC44A2 Antibody

Target: SLC44A2 (Solute Carrier Family 44, Member 2 (SLC44A2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC44A2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SLC44A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%) Gibbon, Monkey, Marmoset (94%) Orangutan, Galago, Dog (88%) Rat, Hamster, Elephant, Panda, Horse, Pig, Guinea pig (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from internal region of human SLC44A2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Gibbon, Monkey, Marmoset (94%), Orangutan, Galago, Dog (88%), Rat, Hamster, Elephant, Panda, Horse, Pig, Guinea pig (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SLC44A2

Target: SLC44A2 (Solute Carrier Family 44, Member 2 (SLC44A2))

Alternative Name: SLC44A2 / CTL2 (SLC44A2 Products)

Synonyms: CTL2 antibody, PP1292 antibody, 1110028E10Rik antibody, RGD1309680 antibody, slc44a2 antibody, ctl2 antibody, MGC78933 antibody, SLC44A2 antibody, DKFZp469A0714 antibody, fc26c03 antibody, wu:fc26c03 antibody, solute carrier family 44 member 2 antibody, solute carrier family 44, member 2 antibody, solute carrier family 44 (choline transporter), member 2 L homeolog antibody, Choline transporter-like protein 2 antibody, solute carrier family 44 (choline transporter), member 2 antibody, SLC44A2 antibody, Slc44a2 antibody, slc44a2.L antibody, ctl2 antibody, slc44a2 antibody, Corgl_0495 antibody

Background: Name/Gene ID: SLC44A2
Subfamily: Choline transporter-like
Family: Transporter

Synonyms: SLC44A2, CTL2, PP1292

Gene ID: 57153

Pathways: SARS-CoV-2 Protein Interactome