BMP2 antibody

Catalog No. ABIN1100286

Product Details anti-BMP2 Antibody

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human BMP-2

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human BMP-2.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7430 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. ELISA: To detect hBMP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BMP-2 (LS-C104469) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBMP-2. Western Blot: To detect hBMP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for BMP2

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Alternative Name: BMP2 / BMP-2 (BMP2 Products)

Synonyms: BDA2 antibody, BMP2A antibody, AI467020 antibody, Bmp2a antibody, BMP-2 antibody, xBMP-2 antibody, xbmp2 antibody, BMP2 antibody, bmp2a antibody, bmp2 antibody, wu:fc59d09 antibody, bone morphogenetic protein 2 antibody, bone morphogenetic protein 2 L homeolog antibody, Bone morphogenetic protein 2 antibody, bone morphogenetic protein 2a antibody, BMP2 antibody, Bmp2 antibody, bmp2.L antibody, bmp2 antibody, bmp2a antibody

Background: Name/Gene ID: BMP2
Family: TGF beta

Synonyms: BMP2, BDA2, BMP-2, BMP-2A, BMP2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A

Gene ID: 650

UniProt: P12643

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation