Caspase 3 antibody (AA 101-112)

Catalog No. ABIN1100424

Product Details anti-Caspase 3 Antibody

Target: Caspase 3 (CASP3)

Binding Specificity: AA 101-112

Reactivity: Human, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human Caspase 3. This antibody is expected to recognise both reported isoforms (NP_004337.2 and NP_116786.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Hamster (100%) Orangutan, Monkey, Galago, Marmoset, Sheep, Horse (92%) Mouse, Rat, Bat, Dog, Bovine, Panda, Rabbit, Porcine, Guinea pig (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RDVSKEDHSKRS from an internal region of human Caspase 3 (NP_004337.2, NP_116786.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Hamster (100%), Orangutan, Monkey, Galago, Marmoset, Sheep, Horse (92%), Mouse, Rat, Bat, Dog, Bovine, Panda, Rabbit, Porcine, Guinea pig (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 30kD band observed in lysates of cell line MOLT4 (calculated MW of 31.6kD according to NP_004337.2,). Recommended concentration: 1-3 μg/mL. An additional band of unknown identity was also consistently observed at 65kD. This band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3 / Caspase 3 (CASP3 Products)

Synonyms: CPP32 antibody, CPP32B antibody, SCA-1 antibody, A830040C14Rik antibody, AC-3 antibody, Apopain antibody, CC3 antibody, Caspase-3 antibody, Lice antibody, Yama antibody, mldy antibody, xcpp32 antibody, casp3 antibody, zgc:100890 antibody, CASP-3 antibody, caspase-3 antibody, caspase 3 antibody, caspase 3 S homeolog antibody, caspase 3, apoptosis-related cysteine peptidase a antibody, caspase 3, apoptosis-related cysteine peptidase antibody, CASP3 antibody, Casp3 antibody, casp3.S antibody, casp3a antibody

Background: Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama

Gene ID: 836

NCBI Accession: NP_004337, NP_116786

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation