GDF15 antibody (HRP)

Catalog No. ABIN1101717

Product Details anti-GDF15 Antibody (HRP)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GDF15 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Specificity: This antibody reacts with endogenous NAG-1 protein from mouse and human tissues. A BLAST analysis suggests reactivity with NAG-1 from rat based on 100 % homology. Partial reactivity is expected against swine, bovine and dog based on 92 % homology. Cross-reactivity with NAG-1 from other sources has not been determined.

Purification: Protein A purified

Immunogen: This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E. coli corresponding to full-length mouse NAG-1 protein. Cross reactivity to MBP was removed via cross-adsorption chromatography.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB

Usage: This Protein-A purified, MBP-cross-adsorbed antibody is suitable for ELISA and western blotting of mouse and human NAG-1 protein. For detection of NAG-1 in mouse serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal) specific antibodies. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of approximately 14 and 28 kD in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA, 0.01 % Gentamicin sulfate

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for GDF15

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15 (GDF15 Products)

Synonyms: GDF15 antibody, GDF-15 antibody, MIC-1 antibody, MIC1 antibody, NAG-1 antibody, PDF antibody, PLAB antibody, PTGFB antibody, SBF antibody, growth differentiation factor 15 antibody, GDF15 antibody, Gdf15 antibody

Background: Name/Gene ID: GDF15
Family: TGF beta

Synonyms: GDF15, MIC1, MIC-1, NAG-1, NRG-1, NSAID-regulated gene 1 protein, PLAB, NSAID-activated gene 1 protein, Placental TGF-beta, PTGFB, GDF-15, PTGF-beta

Gene ID: 9518

UniProt: Q99988

Pathways: SARS-CoV-2 Protein Interactome