NYS48/HAUS8 antibody (pSer70)
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- Target See all NYS48/HAUS8 (HAUS8) Antibodies
- NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))
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Binding Specificity
- pSer70
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NYS48/HAUS8 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Specificity
- Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. The antibody is specific for the phosphorylated form of Hice1. Reactivity with non-phosphorylated human Hice1 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with Hice1 from human based on 100 % sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
- Purification
- Protein A purified
- Immunogen
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Anti-Hice1 pS70 Antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 70 of Hice1.
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product HAUS8 Primary Antibody
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- Application Notes
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Approved: ELISA, WB (1.22 μg/mL)
Usage: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kD in size corresponding to human phosphorylated Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- avoid freeze thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Target
- NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))
- Alternative Name
- HAUS8 (HAUS8 Products)
- Synonyms
- 2410004L22Rik antibody, Hice1 antibody, NY-SAR-48 antibody, hice1 antibody, DGT4 antibody, HICE1 antibody, Ny-sar-48 antibody, RGD1311565 antibody, 4HAUS augmin-like complex, subunit 8 antibody, HAUS augmin like complex subunit 8 L homeolog antibody, HAUS augmin like complex subunit 8 antibody, HAUS augmin-like complex, subunit 8 antibody, Haus8 antibody, haus8.L antibody, HAUS8 antibody
- Background
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Name/Gene ID: HAUS8
Synonyms: HAUS8, DGT4, NY-SAR-48, Sarcoma antigen NY-SAR-48, HICE1 - Gene ID
- 93323
- UniProt
- Q9BT25
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