NCKAP1 antibody (AA 703-715)

Catalog No. ABIN1103200

Product Details anti-NCKAP1 Antibody

Target: NCKAP1 (NCK Associated Protein 1 (NCKAP1))

Binding Specificity: AA 703-715

Reactivity: Human, Mouse, Rat, Cow, Dog, Bat, Chicken, Guinea Pig, Hamster, Horse, Monkey, Rabbit, Xenopus laevis, Zebrafish (Danio rerio)

Host: Goat

Clonality: Polyclonal

Conjugate: This NCKAP1 antibody is un-conjugated

Application: ELISA

Specificity: Human NCKAP1. This antibody is expected to recognize both reported isoforms (NP_038464.1, NP_995314.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Porcine, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Salmon, Stickleback, Medaka, Pufferfish, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-TPREYLTSHLEIR from an internal region of human NCKAP1 (NP_038464.1, NP_995314.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Porcine, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Salmon, Stickleback, Medaka, Pufferfish, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:128000)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave an approx 100kD band in Human Brain (Cerebellum) lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 129kD according to NP_038464.1. The 100kD band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NCKAP1

Target: NCKAP1 (NCK Associated Protein 1 (NCKAP1))

Alternative Name: NCKAP1 / NAP125 (NCKAP1 Products)

Synonyms: HEM2 antibody, NAP1 antibody, NAP125 antibody, p125Nap1 antibody, C79304 antibody, H19 antibody, Hem-2 antibody, Hem2 antibody, Nap1 antibody, mKIAA0587 antibody, mh19 antibody, NCK associated protein 1 antibody, NCK-associated protein 1 antibody, NCK-associated protein 1 L homeolog antibody, NCKAP1 antibody, Nckap1 antibody, nckap1.L antibody

Background: Name/Gene ID: NCKAP1

Synonyms: NCKAP1, HEM2, KIAA0587, NAP1, NAP125, p125Nap1, NAP 1, NCK-associated protein 1

Gene ID: 10787

NCBI Accession: NP_038464, NP_995314

UniProt: Q9Y2A7

Pathways: RTK Signaling, Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis