PCNA antibody

Catalog No. ABIN1103511

The Mouse Monoclonal anti-PCNA antibody (Clone PC10 (a-k-a-3F81)) (ABIN1103511) specifically detects PCNA in WB, IHC, FACS, IHC (p) and IP. The antibody is reactive with Human, Vertebrate and Invertebrate samples.

Quick Overview for PCNA antibody (ABIN1103511)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Vertebrate, Invertebrate

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: PC10 (a-k-a-3F81)

Product Details anti-PCNA Antibody

Purification: Affinity purified

Immunogen: Recombinant rat PCNA

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: Flo, IHC, IHC-P (20 μg/mL), IP, WB

Usage: The PC10 (a. k. a. 3F81) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 150 mM sodium chloride, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing and store undiluted.

Storage: 4 °C

Storage Comment: Store at 4°C, avoid freeze-thaw cycles.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA / Cyclin

Background: Name/Gene ID: PCNA

Synonyms: PCNA, Cyclin

Gene ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy