GNRHR antibody (Extracellular Domain, N-Term)

Catalog No. ABIN1107412

Product Details anti-GNRHR Antibody

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Binding Specificity: AA 1-29, Extracellular Domain, N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GNRHR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Sequence: MANSASPEQN QHCSAINNSI PLMQGNLPY

Specificity: This Monoclonal antibody A9E4 reacts with GnRH receptors in the anterior pituitary. A9E4 showed specificity for the synthetic peptide in ELISA and Dot blot, it has a higher affinity for the antigen than the GnRH receptor antibody clone F1G4 (Cat.-No. AM32064PU-N). A9E4 specifically stains human GnRH receptors on frozen tissue of the anterior pituitary. Recognition was supported by Western blotting.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Purified

Immunogen: Synthetic peptide corresponding to the amino acids 1-29 of the Human GnRH receptor extracellular domain conjugated to BSA. Splenocytes were fused with SP2/0 mouse myeloma cells. Stability was established by subcloning four times.

Clone: A9E4

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C

Target Details for GNRHR

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Alternative Name: GnRH Receptor / GNRHR (GNRHR Products)

Synonyms: GNRHR1 antibody, GRHR antibody, HH7 antibody, LHRHR antibody, LRHR antibody, GNRHR antibody, GH1 antibody, Lhrhr antibody, GNRHR1/III antibody, GNRHR2 antibody, xgnrhr antibody, gnrhr3/ii antibody, gnrh-r antibody, gonadotropin releasing hormone receptor antibody, gonadotropin-releasing hormone receptor antibody, GNRHR antibody, Gnrhr antibody, gnrhr antibody, gnrh-r antibody

Background: Gonadotropin releasing hormone (GnRH) receptor is a protein of 54-60 kDa. GnRH receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular and intracellular loops characteristic of G-protein coupled receptors. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinizing hormone (LH) as well as follicle-stimulated hormone (FSH). The receptor contains of seven hydrophobic transmembrane domains connected by hydrophillic extracellular, and intracellular loops characteristic of G-protein coupled receptors.Synonyms: GRHR, GnRH-R, Gonadotropin-releasing hormone receptor

Gene ID: 2798

NCBI Accession: NP_000397

UniProt: P30968