Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
This antibody detects RAD51 (N-term). No cross reactivity with other proteins.
Cross-Reactivity (Details)
Species reactivity (tested):Human, mouse, rat.
Purification
Immunogen affinity purified
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminal of human RAD51A .Remarks: Sequence is different from the related rat sequence by one amino acid.
DNA repair protein RAD51 homolog 1, also known as RAD51A, is a human gene. The Rad51 gene,HsRAD51, is a homolog of RecA of Escherichia coli and functions in recombination and DNA repair. BRCA1 and BRCA2 proteins form a complex with Rad51, and these genes are thought to participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. RAD51 is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis.Synonyms: DNA repair protein RAD51 homolog 1, RAD51A, RECA