Retinoblastoma 1 antibody (AA 612-928)

Catalog No. ABIN1108868

The Mouse Monoclonal anti-Retinoblastoma 1 antibody is suitable to detect Retinoblastoma 1 in samples from Human. It has been validated for WB, IP and IHC (p).

Quick Overview for Retinoblastoma 1 antibody (AA 612-928) (ABIN1108868)

Target: Retinoblastoma 1 (RB1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3H9

Product Details anti-Retinoblastoma 1 Antibody

Binding Specificity: AA 612-928

Specificity: This antibody reacts with retinoblastoma gene product (110-115 kDa).

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein A Sepharose

Immunogen: Recombinant human Rb protein corresponding to amino acids 612-928

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: Retinoblastoma-Associated Protein / RB1

Background: Mutation of the retinoblastoma tumor suppressor gene alone is sufficient to cause retinoblastoma in humans, suggesting that it might play a role in the normal coordination of cell proliferation and cell death. Deletion or mutational inactivation of the retinoblastoma tumor suppressor protein (Rb) is correlated with the genesis of a variety of human cancers including retinoblastoma, osteosarcoma, and carcinomas of the breast, bladder, and lung. Rb protein is phosphorylated by cyclinD-Cdk4/Cdk6 and cyclinA/cyclinE-Cdk2 during the G1/S transition. This phosphorylation causes the inactivation of the growth inhibitory functions of Rb. Rb undergoes phosphorylation and functional inactivation, permitting the cell to proceed into late G1.Synonyms: OSRC, Rb, Retinoblastoma 1, p105-Rb, pRb, pp110

Gene ID: 5925

NCBI Accession: NP_000312

UniProt: P06400

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy