CD36 antibody (FITC)
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- Target See all CD36 Antibodies
- CD36
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD36 antibody is conjugated to FITC
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF)
- Characteristics
- Monoclonal Mouse Anti-Human CD36 FITC, is recommended for use in flow cytometry for identification of platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes.
- Clone
- TR9
- Isotype
- IgG1
- Top Product
- Discover our top product CD36 Primary Antibody
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- Application Notes
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
- Comment
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Fluorescein isothiocyanate (Molecular Probes).
- Sample Collection
- 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD36 FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The conjugate is provided in liquid form in buffer containing Antibody Stabilizer Solution, 1% bovine serum albumin (BSA), and 0, 09% Sodium azide, pH 7.2.
- Preservative
- Sodium azide
- Precaution of Use
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- Storage
- 4 °C
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- Target
- CD36
- Alternative Name
- CD36 (CD36 Products)
- Synonyms
- BDPLT10 antibody, CHDS7 antibody, FAT antibody, GP3B antibody, GP4 antibody, GPIV antibody, PASIV antibody, SCARB3 antibody, Fat antibody, Scarb3 antibody, GPIIIB antibody, PAS-4 antibody, zgc:92513 antibody, CD36 molecule antibody, CD36 molecule (thrombospondin receptor) antibody, CD36 antibody, Cd36 antibody, cd36 antibody
- Background
- The antibody TR9 cross-blocks binding of FITC-labeled standard antibody OKM5. CD36 is cell adhesion molecule in platelet in adhesion and aggregation, platelet-monocyte and platelet-tumor cell interation. CD36 is expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes. Anti-CD36 antibodies clone TR9, inhibit adhesion function (e.g. adherence of infected erythrocytes to target cells).
- Pathways
- TLR Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism, S100 Proteins
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