CD45RB antibody

Catalog No. ABIN1112467

Product Details anti-CD45RB Antibody

Target: CD45RB

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD45RB antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Monoclonal Anti-Mouse CD45RB is recommended for use in flow cytometry for identification of CD45RB, an isoform of CD45. The C363-16A react with an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. It plays a critical role in TCR and BCR signaling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. Additionally, functionally distinct CD4+ T cell subsets, which secrete differing cytokine profiles, can be separated by CD45RB intensity. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4 and Thy-1. The anti-mouse CD45RB monoclonal antibody reacts with the CD45 isoform containing the exon B dependent epitope. CD45RB is highly expressed on peripheral B cells, cytotoxic T cells, a subset of T helper cells and most thymoctyes.

Immunogen: Cloned mouse Th2 cell lines.

Clone: C363-16A

Isotype: IgG2a

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is less or equal to 1 µg per 10^6 cells in 100 µL volume.

Comment:

Purified, unconjugated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing PBS, 1% BSA and 0.09 % Sodium azide, pH 7,4.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD45RB

Target: CD45RB

Alternative Name: CD45RB (CD45RB Products)

Synonyms: B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, PTPRC antibody, Ptprc antibody

Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions.