MAP2 antibody

Catalog No. ABIN111291

Product Details anti-MAP2 Antibody

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reactivity: Human, Mouse, Rat, Cow, Chicken, Xenopus laevis, Quail

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Thisantibody recognizes (Mr300 kDa) MAP-2 protein from bovine brain. The antibody strongly cross-reacts with MAP-2 from Human, Rat, Mouse, Xenopus, quail and Chicken brains. BM2472 reacts with the High Molecular Weight forms (2a & 2b) of MAP-2 but not with the Low Molecular Weight (70 kDa) form (2c). It reacts with dendrites and cell bodies of neurons.

Purification: Protein G Chromatography.

Immunogen: Bovine brain Microtubule Associated Protein 2.

Clone: AP20

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 1/500. Immunofluorescence: 1/500. Western Blotting: 1-2 μg/mL for 2hrs at RT. Immunohistology (Formalin/Paraffin): 1-2 μg/mL for 30 min at RT. Staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL (OD280)

Buffer: 0.01 M phosphate buffered saline (PBS) pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Aazide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-MAP2 antibody (ABIN111291)

Pan, Tibbe, Harms, Reißner, Becker, Dingmann, Mirzaa, Kattentidt-Mouravieva, Shoukier, Aggarwal, Missler, Kutsche, Kreienkamp: "Missense mutations in CASK, coding for the calcium-/calmodulin-dependent serine protein kinase, interfere with neurexin binding and neurexin-induced oligomerization." in: Journal of neurochemistry, Vol. 157, Issue 4, pp. 1331-1350, (2021) (PubMed).

Target Details for MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Alternative Name: MAP-2 (MAP2 Products)

Synonyms: MAP2A antibody, MAP2B antibody, MAP2C antibody, MAP2R antibody, Mtap2 antibody, MAP-2 antibody, Mtap-2 antibody, xmap2 antibody, MGC53131 antibody, a730034c02 antibody, zgc:103474 antibody, zgc:113974 antibody, microtubule associated protein 2 antibody, microtubule-associated protein 2 antibody, microtubule associated protein 2 S homeolog antibody, MAP2 antibody, Map2 antibody, map2.S antibody, map2 antibody

Background: MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2, two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.Synonyms: MAP2, Microtubule-associated protein 2, Neuronal Marker

Gene ID: 4133

NCBI Accession: NP_002365

UniProt: P11137