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CD93 antibody

CD93 Reactivity: Human FACS, IHC (fro) Host: Mouse Monoclonal X-2 unconjugated
Catalog No. ABIN111895
  • Target See all CD93 Antibodies
    CD93
    Reactivity
    • 55
    • 26
    • 23
    • 4
    Human
    Host
    • 45
    • 15
    Mouse
    Clonality
    • 46
    • 14
    Monoclonal
    Conjugate
    • 20
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This CD93 antibody is un-conjugated
    Application
    • 35
    • 24
    • 13
    • 12
    • 11
    • 11
    • 7
    • 6
    • 6
    • 6
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    Monoclonal antibody X-2 recognizes a 110 kDa O-sialoglycoprotein on granulocytes, monocytes and endothelial cells. Upon differentiation of cultivated monocytes into macrophages, CDw93 ceases to be expressed on day 5-6, which makes it a very useful marker to differentiate those two cell populations. Antigen distribution on isolated cells: Positive with all granulocytes, with staining increasing transiently upon maturation. Antigen expression is increased by TNFα, GM-CSF, (fMLP) treatment. No effect on superoxide production by binding to neutrophils has been noted. PMA stimulation causes down regulation of the antigen. Positive with monocytes following fMLP, TNF, GM-CSF treatment. Antigen expression decreases on cultured monocytes. Positive on AML cases with monocytic component. Positive on cell lines HL-60, Namalva, K562, THP-1, U937, negative on HMC-1 (human mast cell line). Antigen distribution on tissue sections: Monoclonal antibody X-2 stains epitheloid cells and Rosai-Dorfman cells and most endothelial cells in the synovial membrane of RA patients.
    Purification
    Affinity chromatography.
    Immunogen
    Human monocytes
    Clone
    X-2
    Isotype
    IgG1
    Top Product
    Discover our top product CD93 Primary Antibody
  • Application Notes
    Tested for immunohistochemistry (IHC, Frozen sections: 2 μg/mL (1: 100), Does not react onroutinely processed paraffin sections. Suggested positive control: Human tonsil. Has been described to work in FACS.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocol
    Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 0.5 mL distilled water.
    Concentration
    0.2 mg/mL IgG
    Buffer
    PBS, pH 7.2 with 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % Thimerosal as a preservative.
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the antibody at -20 °C. Ship at 2-8 °C.
  • Target
    CD93
    Alternative Name
    CD93 / C1QR1 (CD93 Products)
    Synonyms
    6030404G09Rik antibody, AA145088 antibody, AA4.1 antibody, AW555904 antibody, C1qr1 antibody, C1qrp antibody, Ly68 antibody, C1QR1 antibody, C1qR(P) antibody, C1qRP antibody, CDw93 antibody, ECSM3 antibody, MXRA4 antibody, dJ737E23.1 antibody, C1qRp antibody, CD93 antigen antibody, CD93 molecule antibody, Cd93 antibody, CD93 antibody
    Background
    CD93 a cell-surface glycoprotein and type I membrane protein involve in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.Synonyms: C1q receptor, C1q/MBL/SPA receptor, CDw93, Complement component 1 q subcomponent receptor 1, Complement component C1q receptor, MBL receptor, MXRA4, Matrix-remodeling-associated protein 4, SPA receptor
    Gene ID
    22918
    NCBI Accession
    NP_036204
    UniProt
    Q9NPY3
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