APOB
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
This Apolipoprotein B-48/100 antibody can be used to detect the existence of ApoB-48/100 in plasma and lipoproteins, for immunoassay (1/10,000-1/80,000),Immunoblotting (1/200-1/10,000), Enzyme Conjugation or Biotinylation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL (OD280nm, E0.1% = 1.35)
Buffer
25 mM PBS, 0.15 M NaCl, pH 7.4 containing 1 mM EDTA and 0.02 % Sodium Azide.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 516 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800 mg/L.Synonyms: Apo-B, ApoB, ApoB-100, ApoB100