C3 antibody (HRP)

Catalog No. ABIN113306

Product Details anti-C3 Antibody (HRP)

Target: C3 (Complement Component 3 (C3))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This C3 antibody is conjugated to HRP

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody detects Complement 3c, a polypeptide fragment which is present in C3.

Purification: Affinity chromatography on Protein G.

Immunogen: Complement 3c.

Application Details

Application Notes: Suitable for ELISA (1/200-1/800) and Western blotting (1/500-1/2000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2, containing 0.01 % Thiomersal as preservative.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized product at 2 - 8 °C. Following reconstitution store at -20 °C.

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: Complement C3 (C3 Products)

Synonyms: AHUS5 antibody, ARMD9 antibody, ASP antibody, C3a antibody, C3b antibody, CPAMD1 antibody, AI255234 antibody, HSE-MSF antibody, Plp antibody, C3 antibody, C3-1 antibody, sb:cb26 antibody, si:dkey-76b14.4 antibody, wu:fi34b03 antibody, wu:fj86e11 antibody, C3d antibody, asp antibody, cpamd1 antibody, c3 antibody, complement C3 antibody, complement component 3 antibody, complement component c3a, duplicate 1 antibody, complement component 3 L homeolog antibody, complement component C3 antibody, complement C3-like antibody, C3 antibody, c3a.1 antibody, c3.L antibody, LOC443475 antibody, c3 antibody, c3-1 antibody, LOC100060539 antibody

Background: C3 plays a central role in the activation of the complement system. Processing of C3 by C3 convertase is the central reaction in both classical and alternative complement pathways, resulting in C3a and C3b. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates, and help to initiate the complement cascade, potentially resulting in cellular apoptosis. C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c and C3dg. Defects in C3 can result in susceptibility to pyogenic infection.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3

Gene ID: 718

NCBI Accession: NP_000055

UniProt: P01024

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling