C5 antibody

Catalog No. ABIN113312

Product Details anti-C5 Antibody

Target: C5 (Complement Component 5 (C5))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This C5 antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunodiffusion (ID)

Specificity: Gives a single arc when tested by IEP against fresh Human plasma. Identity has been confirmed by Double Diffusion against fresh Human plasma and a known anti-Human C5.

Purification: Ion Exchange Chromatography

Immunogen: Human C5 purified from plasma

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry: 1/20-1/200 (as a guide). Immunodiffusion.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Glycine buffered saline, pH 7.4, containing 0.09 % Sodium Azide, 0.1 % EACA, 0.01 % Benzamidine and 1 mM EDTA

Preservative: Sodium azide, Benzamidine, EACA

Precaution of Use: This product contains Sodium azide, Benzamidine and EACA: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid multiple freeze/thaw cycles.

Target Details for C5

Target: C5 (Complement Component 5 (C5))

Alternative Name: Complement C5 (C5 Products)

Synonyms: C5 antibody, LOC565774 antibody, C5a antibody, C5b antibody, CPAMD4 antibody, Hc antibody, RGD1561905 antibody, He antibody, complement C5 antibody, complement component 5 antibody, hemolytic complement antibody, C5 antibody, c5 antibody, Hc antibody

Background: C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190 kDa glycoprotein consisting of a disulphide linked alpha-chain (111 kDa) and beta-chain (75 kDa). C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, CPAMD4, Complement component 5

Gene ID: 727

NCBI Accession: NP_001726

UniProt: P01031

Pathways: Complement System, Carbohydrate Homeostasis