KIT Ligand antibody
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- Target See all KIT Ligand (KITLG) Antibodies
- KIT Ligand (KITLG)
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This KIT Ligand antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)
- Specificity
- This antibody recognises Murine Stem Cell Factor.
- Purification
- Affinity chromatography.
- Immunogen
- Highly pure (>98%) recombinant mSCF.
- Top Product
- Discover our top product KITLG Primary Antibody
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- Application Notes
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mSCF (25.0 ng/mL),a concentration of 0.60-0.65 μg/mL of this antibody is required. ELISA: To detect mSCF by direct ELISA (using 100 l/well antibody solution) a concentration of atleast 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant mSCF. Western Blot: To detect mSCF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2 without preservatives.
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- Target
- KIT Ligand (KITLG)
- Alternative Name
- KITLG / SCF (KITLG Products)
- Synonyms
- Xkl-1 antibody, Xsl antibody, Xsl-1 antibody, Xsl-2 antibody, steel antibody, KITLG antibody, SCF antibody, kitl antibody, kl-1 antibody, mgf antibody, scf antibody, FPH2 antibody, KL-1 antibody, Kitl antibody, MGF antibody, SF antibody, SHEP7 antibody, Clo antibody, Con antibody, Gb antibody, Kitlg antibody, Mgf antibody, SLF antibody, Sl antibody, blz antibody, contrasted antibody, 710-712 antibody, CSF antibody, KITL antibody, KIT ligand L homeolog antibody, KIT ligand antibody, kit ligand antibody, kitlg.L antibody, KITLG antibody, kitlg antibody, Kitl antibody, Kitlg antibody
- Background
- Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand
- Gene ID
- 17311
- NCBI Accession
- NP_038626
- UniProt
- P20826
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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