FGF2 antibody (Biotin)
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- Target See all FGF2 Antibodies
- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This FGF2 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificity
- Human Fibroblast Growth Factor basic (FGF-2).
- Purification
- Affinity chromatography.
- Immunogen
- Highly pure (>98%) recombinant hFGF-basic (human FGF-basic).
- Top Product
- Discover our top product FGF2 Primary Antibody
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- Application Notes
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ELISA: To detect hFGF-basic by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthFGF-basic. Western Blot: To detect hFGF-basic by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFGF-basic is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2 without preservatives.
- Preservative
- Without preservative
- Handling Advice
- Centrifuge vial prior to opening!
- Storage
- 4 °C
- Storage Comment
- Store the antibody at 2-8 °C. Do not freeze!
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- Target
- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
- Alternative Name
- FGF Basic / FGF2 (FGF2 Products)
- Synonyms
- BFGF antibody, FGF-2 antibody, FGFB antibody, HBGF-2 antibody, Fgf-2 antibody, Fgfb antibody, bFGF antibody, fibroblast growth factor 2 antibody, fibroblast growth factor 2 (basic) antibody, FGF2 antibody, Fgf2 antibody, fgf2 antibody
- Background
- FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.Synonyms: BFGF, FGFB, Fibroblast growth factor 2 (basic), HBGF-2, HBGF2, Heparin-binding growth factor 2
- Gene ID
- 2247
- NCBI Accession
- NP_001997
- UniProt
- P09038
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins
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