IL-11 antibody

Catalog No. ABIN116103

Product Details anti-IL-11 Antibody

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-11 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: This antibody detects IL11.

Purification: Affinity Chromatograophy employing immobilized hIL-11 matrix

Immunogen: Highly pure (>98%) recombinant hIL-11. Remarks: Molecular Weight: ~150 kDa

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhIL-11 (1.50 ng/mL), a concentration of 0.02-0.05 μg/mL of this antibody is required. Sandwich ELISA: To detect Human IL-11 by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated anti IL11 antibody (PP1033B1 orPP1033B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human IL-11. Western Blot: To detect Human IL-11 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-11 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable for one year from date of receipt at -20 °C. The reconstituted antibody is stable for one month at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 12 months

Target Details for IL-11

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Alternative Name: Interleukin-11 / IL11 (IL11 Products)

Synonyms: IL11 antibody, AGIF antibody, IL-11 antibody, Il-11 antibody, interleukin 11 antibody, IL11 antibody, Il11 antibody

Background: Interleukin 11 is a pleiotropic cytokine produced by mesenchymal-derived adherent cells. IL11 shares many functions of IL6 and LIF, including potentiation of megakaryocyte activity, enhancement of human myeloma cell proliferation, and enhancement of hepatic acute phase protein production. Interleukin 11 directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production. Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelosuppressive chemotherapy.Synonyms: AGIF, Adipogenesis inhibitory factor, IL-11

Gene ID: 3589

NCBI Accession: NP_000632

UniProt: P20809

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion