Direct ELISA: To detect Human PDGF-BB by direct ELISA (using 100 μL/well antibodysolution) this antibody can be used at a concentration of approx. 1.0 μg/mL. This Biotinylatedantibody allows the detection of at least 0.2 ng/well of recombinant hPDGF-BB. Sandwich ELISA: To detect Human PDGF-BB by Sandwich ELISA (using 100 μL/well antibodysolution) this antibody can be used at a concentration of 0.25-1.0 μg/mL. This Biotinylatedantibody, in conjunction with anti-Human PDGF-BB antibody (PP1062P1 or PP1062P2) as aCapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinanthPDGF-BB. Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Target
PDGFB
(Platelet Derived Growth Factor Subunit B (PDGFB))
Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B, the dimers being connected by disulfide bonds. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.Synonyms: Becaplermin, PDGF B, PDGF beta, PDGF subunit B, PDGF-2, PDGF-B, PDGF2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta, Platelet-derived growth factor subunit B, SIS, c-Sis