Osteoprotegerin antibody

Catalog No. ABIN116322

Product Details anti-Osteoprotegerin Antibody

Target: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteoprotegerin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: Recognizes Human Osteoprotegerin (hOPG). Other species not tested.

Purification: Affinity Chromatography employing immobilized hOPG matrix

Immunogen: Highly purified (>98%) E. Coli derived recombinant Human OPG. Remarks: Molecular Weight: 150 kDa

Application Details

Application Notes: Sandwich ELISA: To detect Human OPG by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated anti-Human OPG antibody (PP1107B1 orPP1107B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human OPG. Western Blot To detect Human OPG by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human OPG is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry: This antibody stained Formalin-Fixed Paraffin-Embedded Sectionsof Human breast invasive ductal carcinoma. Use at 0.5 μg/mL with overnight incubation at 4 °C. An HRP-labeled polymer detectionsystem was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0Sodium Citrate buffer is recommended.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for Osteoprotegerin

Target: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Alternative Name: Osteoprotegerin / TNFRSF11B (TNFRSF11B Products)

Synonyms: CD254 antibody, ODF antibody, OPGL antibody, OPTB2 antibody, RANKL antibody, TRANCE antibody, hRANKL2 antibody, sOdf antibody, OCIF antibody, OPG antibody, TR1 antibody, Opg antibody, ocif antibody, opg antibody, tr1 antibody, TNF superfamily member 11 antibody, TNF receptor superfamily member 11b antibody, tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin) antibody, TNF receptor superfamily member 11B antibody, tumor necrosis factor receptor superfamily member 11b S homeolog antibody, tumor necrosis factor receptor superfamily, member 11b antibody, TNFSF11 antibody, TNFRSF11B antibody, Tnfrsf11b antibody, tnfrsf11b.S antibody

Target Type: Chemical

Background: Osteoprotegerin (OPG) is a member of the TNF receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand (TNFSF11/OPGL), both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of the gene have been reported, but their full length nature has not been determined.Synonyms: OCIF, OPG, Osteoclastogenesis inhibitory factor, Tumor necrosis factor receptor superfamily member 11B

Gene ID: 4982

NCBI Accession: NP_002537

UniProt: O00300