TWEAK antibody

Catalog No. ABIN116358

Product Details anti-TWEAK Antibody

Target: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TWEAK antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: Human TWEAK

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hTWEAK.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTWEAK (200 ng/mL), a concentration of 0.1 - 0.2 μg/mL of this antibody is required. ELISA: To detect hTWEAK by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hTWEAK. Western Blot: To detect hTWEAK by Western Blot analysis this antibody can be used at aconcentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTWEAK is 1.5-3.0 ng/lane, under either reducing or ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for TWEAK

Target: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Alternative Name: TWEAK / TNFSF12 (TNFSF12 Products)

Synonyms: APO3L antibody, DR3LG antibody, TWEAK antibody, Apo3l antibody, Dr3l antibody, Dr3lg antibody, Tweak antibody, TNF superfamily member 12 antibody, tumor necrosis factor (ligand) superfamily, member 12 antibody, TNFSF12 antibody, Tnfsf12 antibody

Background: Synonyms: APO3 ligand, APO3L, DR3LG, TNF-related weak inducer of apoptosis, Tumor necrosis factor ligand superfamily member 12

Gene ID: 8742

NCBI Accession: NP_003800

UniProt: O43508

Pathways: Apoptosis