CD137 antibody (Biotin)

Catalog No. ABIN116396

Product Details anti-CD137 Antibody (Biotin)

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD137 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts with 4-1BB Receptor.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant h4-1BB Receptor.

Application Details

Application Notes: ElISA: (Direct: To detect h4-1BB Receptor by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant h4-1BB Receptor. (Sandwich): To detect h4-1BB Receptor by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylatedpolyclonal antibody, in conjunction with Polyclonal Anti-Human 4-1BB Receptor PP1201P asa capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant h4-1BBReceptor. Western Blot: To detect h4-1BB Receptor by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant h4-1BB Receptor is 1.5-3.0 ng/lane, under

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CD137

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Alternative Name: CD137 / TNFRSF9 (TNFRSF9 Products)

Synonyms: TNFRSF9 antibody, zgc:136557 antibody, 4-1BB antibody, CD137 antibody, CDw137 antibody, ILA antibody, A930040I11Rik antibody, AA408498 antibody, AI325004 antibody, Cd137 antibody, Ly63 antibody, tumor necrosis factor receptor superfamily member 9 antibody, TNF receptor superfamily member 9 antibody, tumor necrosis factor receptor superfamily, member 9a antibody, tumor necrosis factor receptor superfamily, member 9 antibody, TNFRSF9 antibody, tnfrsf9a antibody, Tnfrsf9 antibody

Background: CD137 exists on the cell surface as a monomer with a molecular mass of 30 kDa and as a dimer of 55 kDa. Human and mouse CD137 share 60 % amino acid identity. CD137 (4-1BB), a member of the tumour necrosis factor receptor superfamily, is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes. The functions of CD137 in T lymphocytes include regulating activation, proliferation and apoptosis. CD137 and CD28 are costimulatory molecules of T cell activation. Costimulatory molecules are important in initiating anti-tumor immune responses. CD137 plays an important role in regulating T-cell-dependent immune responses. Expression of CD137 correlates negatively with lymphocyte proliferation and positively with the degree of activation-induced cell death caused by mitogen overstimulation. In monocytes, CD137 induces activation, promotes adherence and prolongs survival.Synonyms: 4-1BB homolog, 4-1BB ligand receptor, CDw137, ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9

Gene ID: 3604

NCBI Accession: NP_001552

UniProt: Q07011

Pathways: Cancer Immune Checkpoints