CD137 antibody

Catalog No. ABIN116398

Product Details anti-CD137 Antibody

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD137 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts with 4-1BB Receptor.

Purification: Affinity Chromatography employing immobilized h4-1BB Receptor.

Immunogen: Highly pure (>98%) recombinant Human 4-1BB Receptor.

Application Details

Application Notes: Neutrlization. ELISAIndirect: To detect Human 4-1BB Receptor by indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof at least 0.2-0.4 ng/well of recombinant Human 4-1BB Receptor. Sandwich: To detect Human 4-1BB Receptor by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with with Biotinylated Anti-Human 4-1BB Receptor(PP1201B1 or PP1201B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human 4-1BB Receptor. Western BlotTo detect Human 4-1BB Receptor, this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit forrecombinant Human 4-1BB Receptor is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CD137

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Alternative Name: CD137 / TNFRSF9 (TNFRSF9 Products)

Synonyms: TNFRSF9 antibody, zgc:136557 antibody, 4-1BB antibody, CD137 antibody, CDw137 antibody, ILA antibody, A930040I11Rik antibody, AA408498 antibody, AI325004 antibody, Cd137 antibody, Ly63 antibody, tumor necrosis factor receptor superfamily member 9 antibody, TNF receptor superfamily member 9 antibody, tumor necrosis factor receptor superfamily, member 9a antibody, tumor necrosis factor receptor superfamily, member 9 antibody, TNFRSF9 antibody, tnfrsf9a antibody, Tnfrsf9 antibody

Background: 4-1BB Receptor (also known as TNFRSF9, CD137 antigen, T-cell antigen ILA), a member of the TNF superfamily of receptors, is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB Receptor binds to 4-1BBL to provide a co-stimulatory signal for T lymphocytes. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells. The human 4-1BB Receptor gene codes for a 255 amino acid type I transmembrane protein containing a 17 amino acid N-terminal signal sequence, a 169 amino acid extracellular domain, a 27 amino acid transmembrane domain and a 42 amino acid cytoplasmic domain.Synonyms: 4-1BB homolog, 4-1BB ligand receptor, CDw137, ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9

Gene ID: 3604

NCBI Accession: NP_001552

UniProt: Q07011

Pathways: Cancer Immune Checkpoints