IFNB1 antibody

Catalog No. ABIN116507

The Rabbit Polyclonal anti-IFNB1 antibody has been validated for WB, IHC (p), EIA and Func. It is suitable to detect IFNB1 in samples from Human.

Quick Overview for IFNB1 antibody (ABIN116507)

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFNB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-IFNB1 Antibody

Specificity: This antibody detects Interferon-beta (IFN-beta).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Antigen Affinity Chromatography using immobilized IFN-beta matrix

Immunogen: Highly purified (>98%) E.coli derived recombinant Human Inferferon beta

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman IFN-beta (1.0 ng/mL), a concentration of 0.015-0.025 μg/mL of this antibody isrequired. Indirect ELISA: To detect Human IFN-beta by Indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial before opening.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IFNB1

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Alternative Name: IFNB / Interferon beta

Background: The mammalian type I Inteferons (IFN1) are produced in response to viral infection and other inducers. They are divided into alpha and beta subtypes leukocytes and fibroblasts reactivity. The human IFN alphas are encoded by a family of at least 15 different genes, while IFN beta is the unique member of its subtype. There is approximately 50 % amino acid homology between the alpha and beta subtypes. Both IFN subtypes are pleiotropic cytokines and have a similar range of biological activities. Differences between alpha subtypes, and between IFN alpha and betas, are in potency and cell type specific activities. In particular, IFN beta elicits a markedly higher antiproliferation response in some cell types such as, embryonal carcinoma, melanoma and melanocytes than do IFN alphas. Higher potency of IFN beta in treatment of multiple sclerosis and certain cancers has been observed. Type I IFNs signal through binding to a common cell surface receptor. Two chains of the receptor, IFNAR1 and IFNAR2, have been identified. Both chains are necessary for function and in the absence of either there is neither high affinity binding nor biological activity. The intracellular portions of the receptor subunits are bound by tyrosine kinases, Jak1 and Tyk2, members of the Janus kinase family. Upon ligand binding these kinases are activated and phosphorylate members of the STAT family of transcription factors, as well as IFNAR1 and 2.Synonyms: Fibroblast interferon, IFB, IFN-beta, IFNB1

Gene ID: 3456

NCBI Accession: NP_002167

UniProt: P01574

Pathways: JAK-STAT Signaling, TCR Signaling, TLR Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Autophagy, Inflammasome