APOA2 antibody

Catalog No. ABIN116542

Product Details anti-APOA2 Antibody

Target: APOA2 (Apolipoprotein A-II (APOA2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This product has been prepared by Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with ApoLipoprotein A-II and has negligible cross-reactivity with Type A-I, B, C-I, C-II, C-III, E and J apoLipoproteins. Non-specific cross reaction of anti-apoLipoprotein antibodies with other Human serum proteins is negligible.

Purification: Immunoaffinity Chromatography.

Immunogen: ApoLipoprotein Type A-II was isolated from Human plasma by Density Gradient Centrifugation followed by HPLC purification.

Application Details

Application Notes: This Apo AII antibody R1031P has been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and Western blotting forhighly sensitive qualitative analysis. Recommended Dilutions: ELISA: 1/5,000-1/10,000. Immunoprecipitation: 1/100. Western blot: 1/5,000-1/10,000. Immunohistochemistry: 1: /50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 and 0.01 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for APOA2

Target: APOA2 (Apolipoprotein A-II (APOA2))

Alternative Name: Apolipoprotein A II (Apo AII) (APOA2 Products)

Synonyms: Apo-AII antibody, ApoA-II antibody, apoAII antibody, Alp-2 antibody, ApoAII antibody, Apoa-2 antibody, Hdl-1 antibody, APOAII antibody, apoa2 antibody, apoA-II antibody, cb1032 antibody, wu:fb57h11 antibody, zgc:193613 antibody, apolipoprotein A2 antibody, apolipoprotein A-II antibody, APOA2 antibody, Apoa2 antibody, apoa2 antibody

Background: Apolipoprotein A II is the second most abundant protein of the high density lipoprotein particles. The apolipoprotein A II gene consists of 4 exons and 3 introns. The four exons encode the 5' untranslated region, pre peptide, a short N terminal domain and a C terminal domain composed of a variable number of lipid binding amphipathic helices. Familial apolipoprotein A II deficiency may result from a splice junction alteration which blocks splicing of intron 3 from the primary transcript and result in the formation of a non functional mRNA.Synonyms: APOA2, Apo-AII, ApoA-II, Apolipoprotein A-II, Apolipoprotein A2

Gene ID: 336

NCBI Accession: NP_001634

UniProt: P02652

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Negative Regulation of Transporter Activity, Lipid Metabolism