COL4A1 antibody (AA 1444-1669)
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- Target See all COL4A1 Antibodies
- COL4A1 (Collagen, Type IV, alpha 1 (COL4A1))
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Binding Specificity
- AA 1444-1669
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This COL4A1 antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Purpose
- Polyclonal Antibody to Collagen Type IV Alpha 1 (COL4a1)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against COL4a1. It has been selected for its ability to recognize COL4a1 in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Collagen Type IV Alpha 1 (COL4a1) corresdonding to His1444~Thr1669 with N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product COL4A1 Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- COL4A1 (Collagen, Type IV, alpha 1 (COL4A1))
- Alternative Name
- Collagen Type IV Alpha 1 (COL4A1 Products)
- Synonyms
- col4a1 antibody, wu:fc02g11 antibody, HANAC antibody, ICH antibody, POREN1 antibody, arresten antibody, Bru antibody, Col4a-1 antibody, Del(8)44H antibody, Raw antibody, Svc antibody, collagen, type IV, alpha 5 (Alport syndrome) antibody, collagen type IV alpha 1 chain antibody, collagen, type IV, alpha 1 antibody, col4a5 antibody, COL4A1 antibody, Col4a1 antibody
- Background
- COL4-A1, Arresten, Collagen Alpha-1(IV)chain
- Pathways
- Skeletal Muscle Fiber Development, Growth Factor Binding
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