MAPKAP Kinase 2 antibody (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2) (pThr334) (PE)

Details for Product anti-MAPKAPK2 Antibody No. ABIN1177087, Supplier: Log in to see
Antigen
  • mapkapk2
  • wu:fb30e10
  • wu:fc56c10
  • wu:fi03a01
  • MGC78852
  • MAPKAPK2
  • AA960234
  • MAPKAP-K2
  • MK-2
  • MK2
  • Rps6kc1
  • mitogen-activated protein kinase-activated protein kinase 2a
  • mitogen-activated protein kinase-activated protein kinase 2 L homeolog
  • mitogen-activated protein kinase-activated protein kinase 2
  • MAP kinase-activated protein kinase 2
  • mapkapk2a
  • mapkapk2.L
  • MAPKAPK2
  • Mapkapk2
Alternatives
anti-Human MAPKAP Kinase 2 antibody for Western Blotting
Epitope
pThr334
57
32
23
15
15
15
14
10
7
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3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
236
185
142
43
13
9
8
6
5
5
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4
3
2
2
1
Host
Mouse
217
28
15
Clonality (Clone)
Monoclonal ()
Conjugate
This MAPKAP Kinase 2 antibody is conjugated to PE
7
7
7
5
4
4
4
4
3
3
3
3
3
3
3
3
3
Application
Intracellular Staining (ICS)
201
117
104
40
39
39
26
18
7
5
4
2
2
1
Options
Supplier
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Supplier Product No.
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Brand BD Phosflow™
Immunogen Phosphorylated Human MAPKAPK-2 Peptide
Clone P24-694
Isotype IgG1, kappa
Purification Purified from tissue culture supernatant or ascites by affinity chromatography
Plasmids, Primers & others Plasmids, Primers & others MAPKAP Kinase 2 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name MAPKAPK-2 (MAPKAPK2 Antibody Abstract)
Background The P24-694 monoclonal antibody specifically binds to the phosphorylated T334 site (pT334) of MAPKAPK-2. MAPKAPK-2 is a serine/threonine protein kinase. This ~49 kDa member of the MAPKAPK family of protein kinases is also known as mitogen-activated protein kinase-activated protein kinase 2. MAPKAPK-2 is phosphorylated and activated by p38 MAP kinase in response to stress, cytokines and chemokines. MAPKAPK-2 is phosphorylated on multiple sites including Thr222, Ser272 and Thr334. Phosphorylation of any two of these three amino acid residues seems to be required for the activation of this kinase that serves multiple cellular functions. Phosphorylation of Thr334 was reported to be essential for nuclear export of the heterodimer formed between p38 MAPK and MAPKAPK-2. Mice deficient in MAPKAPK-2 have been shown to be protected from ischemic injury. MAPKAPK-2 is also reported to serve as a cell cycle checkpoint kinase in response to UV irradiation. The heat shock protein, HSP27 was shown to be one of the major substrates of MAPK and MAPKAPK-2.
Synonyms: MAP kinase-activated protein kinase 2, MAPKAP kinase 2, MK2, MAPK2
Pathways MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
Sample Volume 5 μL
Restrictions For Research Use only
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product cited in: Manke, Nguyen, Lim, Stewart, Elia, Yaffe: "MAPKAP kinase-2 is a cell cycle checkpoint kinase that regulates the G2/M transition and S phase progression in response to UV irradiation." in: Molecular cell, Vol. 17, Issue 1, pp. 37-48, 2005 (PubMed).

Wang, Xu, Wang, Young, Gaestel, Feuerstein: "Mitogen-activated protein kinase-activated protein (MAPKAP) kinase 2 deficiency protects brain from ischemic injury in mice." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 43968-72, 2002 (PubMed).

Heidenreich, Neininger, Schratt, Zinck, Cahill, Engel, Kotlyarov, Kraft, Kostka, Gaestel, Nordheim: "MAPKAP kinase 2 phosphorylates serum response factor in vitro and in vivo." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14434-43, 1999 (PubMed).

Engel, Kotlyarov, Gaestel: "Leptomycin B-sensitive nuclear export of MAPKAP kinase 2 is regulated by phosphorylation." in: The EMBO journal, Vol. 17, Issue 12, pp. 3363-71, 1998 (PubMed).

Krump, Sanghera, Pelech, Furuya, Grinstein: "Chemotactic peptide N-formyl-met-leu-phe activation of p38 mitogen-activated protein kinase (MAPK) and MAPK-activated protein kinase-2 in human neutrophils." in: The Journal of biological chemistry, Vol. 272, Issue 2, pp. 937-44, 1997 (PubMed).

Ben-Levy, Leighton, Doza, Attwood, Morrice, Marshall, Cohen: "Identification of novel phosphorylation sites required for activation of MAPKAP kinase-2." in: The EMBO journal, Vol. 14, Issue 23, pp. 5920-30, 1996 (PubMed).

Clifton, Young, Cohen: "A comparison of the substrate specificity of MAPKAP kinase-2 and MAPKAP kinase-3 and their activation by cytokines and cellular stress." in: FEBS letters, Vol. 392, Issue 3, pp. 209-14, 1996 (PubMed).

Rouse, Cohen, Trigon, Morange, Alonso-Llamazares, Zamanillo, Hunt, Nebreda: "A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins." in: Cell, Vol. 78, Issue 6, pp. 1027-37, 1994 (PubMed).

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